Obtaining mode and application of borrelia burgdorferi flagellin FlaB antibody

A flagellin and Borrelia technology, applied in application, immunoglobulin, antibacterial immunoglobulin, etc., can solve the problem of not yet developed an effective vaccine, and achieve the effect of saving antigen, high titer, and time.

Inactive Publication Date: 2019-01-04
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

An effective vaccine ha

Method used

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  • Obtaining mode and application of borrelia burgdorferi flagellin FlaB antibody
  • Obtaining mode and application of borrelia burgdorferi flagellin FlaB antibody
  • Obtaining mode and application of borrelia burgdorferi flagellin FlaB antibody

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Experimental program
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Embodiment Construction

[0033] 1. Research materials

[0034] 1. Experimental animals

[0035] The male Balb / c mice (8-12 weeks old) used in this study were from the Experimental Animal Center of the Institute of Medical Biology, Chinese Academy of Medical Sciences, the experimental animal production license [SCXX (Dian) K2017-0002], the use license [ SYXK (Dian) 2014-0002]. All animal use procedures were reviewed and approved by the Experimental Animal Ethics Committee of Medical Biology, Chinese Academy of Medical Sciences.

[0036] 2. Strains: Borrelia Burgdorferi B31 strain (hereinafter referred to as 4680) and Borrelia hermsii HS1 strain (hereinafter referred to as 5251) were purchased from NTCC.

[0037] 3. Experimental reagents

[0038] 1) Antibody: Anti-mouse IgG antibody (Cell Signaling Technology, Cat#7076P2), AlexaFluor-488 labeled anti-mouse IgG antibody (Abcam, Cat#ab150113).

[0039] 2) Immune adjuvant: QuickAntibody-Mouse5W (Beijing Boaolong Immunotechnology Co., Ltd., Cat#KX021004...

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Abstract

The invention relates to preparation and application of a borrelia burgdorferi flagellin FlaB antibody. By means of a PCR method, a gene segment of FlaB is obtained from a borrelia burgdorferi genomethrough amplification, cloned into a prokaryotic expression vector pET30a containing a histidine tag and then transferred into escherichia coli BL21 competent cells. Induction expression is carried out through IPTG, affinity purification is performed to obtain FlaB protein, immunized mice obtain the borrelia burgdorferi flagellin FlaB antibody, and a solid foundation is laid for detection and deepresearch of borrelia burgdorferi.

Description

technical field [0001] The invention relates to the field of biological immunity, in particular to a method for preparing an antibody to phologellin FlaB, and more specifically to the application of the prepared antibody to detect Borrelia by different immunological methods. technical background [0002] Relapsing fever is an infectious disease caused by Borrelia bacteria infection with lice and ticks as the medium (Schwan, T.G. and J.Piesman, Vector interactions and molecular adaptations of lymedisease and relapsing fever spirochetes associated with transmission byticks. (2): p.115-21; Cutler, S.J., Possibilities for relapsing fever reemergence. Emerg Infect Dis, 2006.12(3): p.369-74). Most patients develop clinical reactions within 5 to 15 days after infection, including sudden cold, hot, headache, muscle or joint pain, and nausea (Ramos, J.M., et al., Results of a 10-year survey of louse -borne relapsing fever in southern Ethiopia: a decline in endemicity.Ann Trop Med Pa...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/06C07K14/20C12N15/31C12N15/70G01N33/569
CPCC07K14/20C07K16/065C07K16/1207C12N15/70G01N33/56911G01N2333/20Y02A50/30
Inventor 刘红旗时宇陆巍李昕潼张静陶玉芬刘建生
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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