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A kind of screen printing electrode for detecting uric acid and preparation method thereof

A technology for screen printing electrodes and electrodes is applied in the field of chitosan-based carbon nanotube-gold composite film modified screen printing electrodes and their preparation, which can solve problems such as interference and achieve low cost, fast response time, and uniform thickness. controllable effect

Active Publication Date: 2017-01-04
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the oxidation peak potential of UA is very close to that of ascorbic acid (AA), uric acid voltammetric sensors are usually interfered by AA

Method used

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  • A kind of screen printing electrode for detecting uric acid and preparation method thereof
  • A kind of screen printing electrode for detecting uric acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Wash the SP electrode with ultrapure water and dry it at room temperature;

[0026] (2) Configure 200mg / L chloroauric acid solution, place the clean SP electrode in the chloroauric acid solution, deposit with -0.2V constant potential for 180s, wash with ultrapure water, and dry to prepare Au / SP electrode;

[0027] (3) Prepare 1.0 wt% CS solution with 0.5% hydrochloric acid solution in volume fraction, stir on a magnetic stirrer for 0.5 h, filter with a vacuum pump, and take the filtrate;

[0028] (4) Disperse 0.01g CNTs in 20mL (3) CS solution, stir well to mix evenly, and sonicate on the cell crusher for 10min to better disperse the carbon nanotubes, and then adjust the pH with 1.0wt% NaOH Is 5.0 to prepare a 0.5 mg / mL chitosan-carbon nanotube dispersion;

[0029] (5) Place the Au / SP electrode prepared in (2) in the chitosan-carbon nanotube dispersion in (4), use -1.2V potential to form a film for 30 seconds, take it out, wash and dry , The CS-CNTs / Au / SP electrode is ob...

Embodiment 2

[0031] (1) Wash the SP electrode with ultrapure water and dry it at room temperature;

[0032] (2) Configure 200mg / L chloroauric acid solution, place the clean SP electrode in the chloroauric acid solution, deposit with -0.3V constant potential for 600s, wash with ultrapure water, and dry to prepare Au / SP electrode;

[0033] (3) Prepare 1.0 wt% CS solution with 0.5% hydrochloric acid solution in volume fraction, stir on a magnetic stirrer for 0.5 h, filter with a vacuum pump, and take the filtrate;

[0034] (4) Disperse 0.01g CNTs in 10mL (3) CS solution, stir well to mix evenly, and sonicate on the cell crusher for 10min to better disperse the carbon nanotubes, and then adjust the pH with 1.0wt% NaOH At 6.0, a 1 mg / mL chitosan-carbon nanotube dispersion was prepared;

[0035] (5) Place the Au / SP electrode prepared in (2) in the chitosan-carbon nanotube dispersion in (4), use -1.5V potential to form a film for 120s, take it out, wash and dry , The CS-CNTs / Au / SP electrode is obtaine...

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Abstract

The invention discloses an electrochemical sensor of a screen-printed electrode modified by chitosan-based carbon nanotube-gold composite film, a preparation method of the screen-printed electrode, and electrochemical detection of the modified electrode for uric acid under a condition that ascorbic acid exists. The preparation method comprises the following steps: cleaning the screen-printed electrode with ultrapure water, and drying in the air at the room temperature; preparing the screen-printed electrode modified by gold by a constant potential deposition method; and adopting constant potential film formation to obtain the screen-printed electrode modified by chitosan-based carbon nanotube-gold. The electrode chip is used for detecting the uric acid under the condition that the ascorbic acid exists. The screen-printed electrode has the advantages of being simple in preparation method, low in cost, rapid in response time, wide in linear range, good in reproducibility and stability, and the like, and can be used for rapidly detecting the uric acid under the condition that the ascorbic acid exists.

Description

Technical field [0001] The invention relates to an electrode for electrochemical detection, in particular to a chitosan-based carbon nanotube-gold composite film modified screen printing electrode and a preparation method thereof. Background technique [0002] Uric acid (UA) is the final product of purine metabolism in the human body. It has been shown that abnormal UA concentration may cause the following diseases: gout, hyperuricemia and Lesch-Nyhan syndrome. In addition, UA as a reducing agent can consume oxygen free radicals and prevent them from damaging tissues and cells. As a daily health check in medical diagnosis, many types of UA sensors have been developed and commercialized. For example, colorimetry, spectroscopy, and chromatography have been widely used in UA detection. Generally, voltammetry technology has the following advantages compared with the above traditional methods: high selectivity, low cost and low time consumption. However, since the oxidation peak po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327
Inventor 施晓文刘桂亭曹发苏晓明
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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