Blood flower milkweed milk extract rich in cardiac glycosides as well as preparation method and application of extract

A technology of milkweed and cardiac glycosides, which is applied in the field of natural medicinal chemistry, can solve problems not involved in the application of milkweed extract, and achieve good anti-tumor activity, convenient extraction, and various types of effects

Inactive Publication Date: 2015-04-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These documents all focus on milkweed, but do not inv...

Method used

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  • Blood flower milkweed milk extract rich in cardiac glycosides as well as preparation method and application of extract
  • Blood flower milkweed milk extract rich in cardiac glycosides as well as preparation method and application of extract
  • Blood flower milkweed milk extract rich in cardiac glycosides as well as preparation method and application of extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of a milkweed milk extract rich in cardiac glycosides

[0039] A, get the milk 1mL of milkweed (solid matter after drying is 100mg), dilute with 5mL distilled water, obtain diluent, add 3mL n-butanol to the diluent and extract, collect the n-butanol layer after extraction; Add 3 mL of n-butanol to the remaining water layer to continue the extraction, and repeat this process for 3 times, discard the water layer, and combine the n-butanol layers to obtain 9 mL of n-butanol extract;

[0040] B. Use the N-1100V-W type rotary evaporator to concentrate the n-butanol extract to constant weight under reduced pressure at 30°C to obtain a white slurry, which is the milkweed milk extract rich in cardiac glycosides (AC -1, 20.5mg, the extraction rate is 20.5%);

[0041] (2) The milkweed milk extract rich in cardiac glycosides obtained in step (1) is analyzed

[0042]The milkweed milk extract rich in cardiac glycosides that step (1) obtains is dissolved in methanol...

Embodiment 2

[0060] (1) Preparation of a milkweed milk extract rich in cardiac glycosides

[0061] A. Take milkweed milk 1mL (dried solid content is 100mg) and dilute it with 7mL distilled water to obtain a diluent; add 5mL n-butanol to the diluent for extraction, collect the n-butanol layer after extraction; Add 5 mL of n-butanol to the lower water layer to continue the extraction, and repeat this for 3 times, discard the water layer, and combine the n-butanol layers to obtain 15 mL of n-butanol extract;

[0062] B. Concentrate the n-butanol extract under reduced pressure at 50°C to constant weight with an N-1100V-W rotary evaporator to obtain a white slurry, which is the milkweed milk extract rich in cardiac glycosides (AC -1, 20.0mg, the extraction rate of this extract is 20.0%);

[0063] (2) the milkweed milk extract rich in cardiac glycosides obtained in step (1) is analyzed

[0064] The milkweed milk extract rich in cardiac glycosides that step (1) obtains is dissolved in methanol,...

Embodiment 3

[0091] (1) Preparation of a milkweed milk extract rich in cardiac glycosides

[0092] A. Take 1mL milkweed milk (dried solid content is 100mg), dilute with 8mL distilled water to obtain a dilution; add 3mL ethyl acetate to the dilution for extraction, and collect the extracted ethyl acetate layer; Add 3 mL of ethyl acetate to the remaining water layer to continue the extraction, and cycle like this for 4 times, discard the water layer, combine the ethyl acetate layers to obtain 12 mL of ethyl acetate extract;

[0093] B. Use the N-1100V-W type rotary evaporator to concentrate the ethyl acetate extract under reduced pressure to constant weight at 30°C to obtain a white slurry, which is the milkweed milk extract rich in cardiac glycosides (AC -1, 20.2mg, the extraction rate is 20.2%);

[0094] (2) The milkweed milk extract rich in cardiac glycosides obtained in step (1) is analyzed

[0095] The milkweed milk extract rich in cardiac glycosides that step (1) obtains is dissolved...

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Abstract

The invention belongs to the chemical field of natural medicine and discloses a blood flower milkweed milk extract rich in cardiac glycosides as well as a preparation method and an application of the extract. The preparation method of the extract comprises the following steps: (1) diluting the milk of the blood flower milkweed by use of water to obtain a diluent; adding an organic solvent to the diluent for extracting, and collecting the organic layer obtained after extraction; next, adding the organic solvent to the left water layer for extracting, collecting the organic layer again, and circularly extracting for 2-4 times, and then abandoning the water layer, and blending the organic the organic layers to obtain the cardiac glycoside extract of the blood flower milkweed milk; (2) performing reduced pressure concentration on the blood flower milkweed milk until a constant weight, thereby obtaining the blood flower milkweed milk extract rich in cardiac glycosides. The blood flower milkweed milk extract rich in cardiac glycosides has excellent anti-tumor activity and low toxicity to normal cells; the extract contains a plurality of types of cardiac glycosides rich in content. The preparation method of the blood flower milkweed milk extract rich in cardiac glycosides is simple, low in cost, high in extraction rate, and high in anti-tumor selectivity.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and particularly relates to a milkweed milk extract rich in cardiac glycosides, a preparation method thereof and an application in antitumor drugs. Background technique [0002] According to a survey report published by the World Health Organization on February 1, 2013, the number of people who died of cancer in the world in 2008 reached 7.6 million, and there were about 13 million new cancer patients every year, of which nearly 60% were lung cancer, gastric cancer, breast cancer, Prostate cancer, colorectal cancer, oral cancer, liver cancer, cervical cancer and esophageal cancer are the second leading causes of death after cardiovascular disease. At present, cancer treatment includes surgery, radiotherapy and chemotherapy, intervention and other means, but the mortality rate is still high. Experts and scholars from all over the world are actively researching to find methods and drugs ...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61K31/585A61P35/00
CPCA61K31/585A61K36/27A61K2236/33A61K2236/39A61K2300/00
Inventor 江仁望张容容谭亚芳田海妍朱自荣
Owner JINAN UNIVERSITY
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