Porcine reproductive and respiratory syndrome (PRRS) egg yolk antibody oral solution and preparation method thereof

A technology of egg yolk antibody and porcine blue-ear disease, which is applied in the direction of antibodies, antiviral agents, and pharmaceutical formulas, can solve the problems of inconvenient use of porcine blue-ear disease egg yolk antibody, and improve immunity, stress ability, and biological activity Unaffected, highly bioavailable effects

Inactive Publication Date: 2015-04-22
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a porcine PRRS yolk antibody oral liquid and a preparation method thereof, which solves the problem of inconvenient use of porcine PRRS yolk antibody for injection

Method used

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  • Porcine reproductive and respiratory syndrome (PRRS) egg yolk antibody oral solution and preparation method thereof
  • Porcine reproductive and respiratory syndrome (PRRS) egg yolk antibody oral solution and preparation method thereof
  • Porcine reproductive and respiratory syndrome (PRRS) egg yolk antibody oral solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A formula for oral solution of yolk antibody against PRRS includes the following raw materials: 95 L of whole egg aqueous solution containing yolk antibody, 0.2 L of caprylic acid, 0.3 L of formaldehyde, 0.02 L of thimerosal, and 1000 units / ml of penicillin and streptomycin in the oral solution.

[0018] Its preparation method comprises the following steps:

[0019] 1) The commercially available inactivated PRRS vaccine was used for booster immunization. The vaccine dosage for each chicken was 2ml each time, and the immunization procedure was carried out three times with an interval of 10 days. When the antibody titer reached 1:256, the collection began High free eggs.

[0020] 2) Sort the collected high-free eggs, sterilize with 0.2% bromogeramine, and set aside.

[0021] 3) Acidification treatment by water dilution method: Beat 2) Bugao egg-free whole eggs into the pasteurized tank for full emulsification. The emulsification time is 7 minutes, and then add the emulsi...

Embodiment 2

[0040] A formula for oral solution of yolk antibody against PRRS includes the following raw materials: 105 L of whole egg aqueous solution containing yolk antibody, 0.2 L of octanoic acid, 0.2 L of formaldehyde, 0.01 L of thimerosal, and 800 units / ml of penicillin and streptomycin in the oral solution.

[0041] Its preparation method comprises the following steps:

[0042] 1) The commercially available inactivated PRRS vaccine was used for booster immunization. The dose of vaccine per chicken was 2ml each time, and the immunization procedure was carried out three times with an interval of 10 days. When the antibody titer reached 1:128, the collection began High free eggs.

[0043] 2) Sort the collected high-free eggs, sterilize with 0.1% bromogeramine, and set aside.

[0044] 3) Acidification treatment by water dilution method: Beat 2) Bugao egg-free whole eggs into the pasteurized tank for full emulsification, emulsification time is 10 minutes, and then add the emulsified wh...

Embodiment 3

[0063] A formula for an oral solution of yolk antibody against PRRS includes the following raw materials: 100 L of whole egg aqueous solution containing yolk antibody, 0.3 L of octanoic acid, 0.3 L of formaldehyde, 0.02 L of thimerosal, and 1000 units / ml of penicillin and streptomycin in the oral solution.

[0064] Its preparation method comprises the following steps:

[0065] 1) The commercially available inactivated PRRS vaccine was used for booster immunization. The vaccine dosage for each chicken was 2ml each time, and the immunization procedure was carried out three times with an interval of 10 days. When the antibody titer reached 1:256, the collection began High free eggs.

[0066] 2) Sort the collected high-free eggs, sterilize with 0.2% bromogeramine, and set aside.

[0067] 3) Acidification treatment by water dilution method: Beat 2) Bugao egg-free whole eggs into the pasteurized tank for full emulsification, emulsification time is 8 minutes, and then add the emulsi...

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Abstract

The invention discloses a porcine reproductive and respiratory syndrome (PRRS) egg yolk antibody oral solution and a preparation method thereof. According to a formula, the PRRS egg yolk antibody oral solution comprises the following raw materials in percentage by volume: 95-105 of a whole-egg water solution containing an egg yolk antibody, 0.2-0.3 of octanoic acid, 0.2-0.3 of formaldehyde, 0.01-0.02 of merthiolate and 800-1000 units of mycillin in every milliliter of the oral solution. Whole eggs with high immunoglobulin are adopted during preparation, so that the clear egg yolk antibody oral solution is obtained. The egg yolk antibody oral solution prepared by using the preparation method is not only low in loss, high in immune active substance content, high in biological activity and free of clinical side effects in the production process, but also contains a great number of various proteins in egg white, so that the adverse effects on the antibody can be reduced during oral administration.

Description

technical field [0001] The invention belongs to the technical field of biological products for pigs, and relates to a pig yolk antibody oral liquid for PRRS and a preparation method thereof. Background technique [0002] Pig blue ear disease, commonly known as porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS), is caused by pig blue ear disease virus, infected pigs have fever, anorexia, late abortion, premature birth, stillbirth, weak Fetal and mummified fetuses, a highly contagious disease characterized by respiratory disturbances in pigs of all ages (especially piglets), is highly contagious and fatal. [0003] Yolk antibody IgY is a kind of immunoglobulin Ig. IgY is functionally equivalent to mammalian IgG, but it is different in structure and has a typical spatial conformation of immunoglobulins. Yolk antibody has many advantages. (1) Due to the phylogenetic gap between birds and mammals, birds are more suitable for prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/00A61P31/14
Inventor 徐进郭俊清
Owner ZHENGZHOU HOUYI PHARMA
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