Method for detecting special growth and development law of muscles of Jingning chicken

Abstract

The invention provides a method for detecting a special growth and development law of muscles of Jingning chicken. The Jingning chicken are unique and peculiar local chicken of the Gansu province, are fresh and tender in texture and unique in flavor, and have good public praise, however, the Jingning chicken are endangered, and the prospect is grim. In the method for detecting the special growth and development law of the muscles of the Jingning chicken, chick embryos of the Jingning chicken are researched, and special meat quality trait controlling genes such as growth and development gap-associated proteins: CECR2 (cat eye syndrome chromosome region) and IGF-1 (insulinlike growth factor-1), fatty acid metabolism gap-associated proteins: Ex-FABP (fatty acid-binding protein) and LPL (lipoprotein lipase) and protein degradation gap-associated proteins: CAST and CAPN1 are selected. Protein expression distribution and change of expression level of proteins in posterior limb buds and body segments in a chick embryo development HH19-31 period are detected by using methods of immunohistochemistry and Western blotting and the like, and the Jingning chicken are compared to white feather broilers, yellow feather broilers and hyline brown chicken. By the method for detecting the special growth and development law of the muscles of the Jingning chicken, the relation of growth and development regulatory functional genes, growth performance and meat quality is discussed, and data are provided for establishing target genes of transgenic chicken. Moreover, the resource superiority can be widely developed and used, and new vitality is injected to development of local economy.

Description

technical field [0001] In the field of chicken muscle quality research, the present invention relates to the field of regulating genes for characteristic meat quality traits, and in particular to a method for detecting the special growth and development rules of Jingning chicken muscle. Background technique [0002] With the development of modern chicken industry and the continuous improvement of people's living standards, the research on chicken muscle quality has become a common concern of scientific and technological workers. However, most of the existing studies only regard the type, density and diameter of muscle fibers as important factors, or study the growth and changes of muscle fibers at different growth and development stages, and the research on the developmental period mainly starts from the newly hatched chicks. Science and technology, especially the experimental technology in the field of life sciences, is changing with each passing day. The existing researc...

AI Technical Summary

Problems solved by technology

Science and technology, especially the experimental technology in the field of life sciences, is changing with each passing day. The existing research methods are only far-fetched to explain the good and bad of muscle quality from a macro perspective, which is far from being used as evidence for the quality of muscle taste. The law of development itself is unsystematic and unscientific