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Preparation method of recombinant organism 3-hydracrylic acid

A technology of hydroxypropionic acid and recombinant microorganisms, applied in the field of biomedicine

Inactive Publication Date: 2015-05-13
XINCHANG GUANYANG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods of production and marketing of 3-HP are relatively expensive, and the manufacture of polymers that use its monomers is expensive to produce
However, a catalog has not yet been provided of the genes from these organisms, whereby the enzymes used to synthesize 3-HP are themselves responsible for the native host production of which is not present in the molecule's synthetic capacity

Method used

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Embodiment Construction

[0014] It was revealed here that it could be introduced into a bacterial host with genes encoding two enzymes, thereby endowing the host with the ability to produce 3-HP from glycerol. The two essential enzymes are glycerol dehydrogenase and acetaldehyde dehydrogenase. It is reported here that these two enzymes are necessary and sufficient to make suitable host strains, such as E. coli strains competent, from glycerol to 3-HP. An exemplary gene encoding glycerol dehydratase is known, DHAB Klebsiella pneumoniae, the gene was sequenced and presented for convenience. Several exemplary aldehyde dehydrogenases are known, and their sequences are presented here. From this information, it becomes possible to confer the ability to actually convert glycerol to 3-HP in a commercially reasonable manner to the bacterial host.

[0015] It was not seen before the work described here that these two distinct enzymes could be manufactured in a common host to produce the ability to make 3-HP. ...

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Abstract

The invention relates to a preparation method of 3-hydracrylic acid, which can be explained by the production of 3-hydracrylic acid (3-HP) from glycerol in a bacterial host. 3-HP is a useful raw material for production of a polymeric material. A genetic engineering bacteria host of two enzymes is enough to produce 3-HP. One enzyme is glycerol dehydratase and the other enzyme is acetaldehyde dehydrogenase. The recombinant microorganism host, which is allowed to be established, can synthesize 3-HP from glycerol or glucose used as a starting material. Glycerol or glucose is converted and then transformed into 3-HP.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing recombinant organism 3-hydroxypropionic acid. Background of the invention [0002] Genetic engineering technology that allows the transfer of genetic traits between species and permits, especially the transfer of enzymes from one species to others. These technologies have reached the commercialization of high value-added products such as pharmaceuticals. The genetic engineering technique is equally applicable and cost-effective when applied to genes and enzymes that can be used to make basic chemical raw materials. [0003] A metabolic pathway is also present in the bacterium Klebsiella pneumoniae, which has the ability to bioproduce 3-hydroxypropane from glycerol. Ability of native microorganisms to produce 1,3-propanediol from glycerol, as well. Commercial interests are exploring the production of 1,3-propanediol from glycerol or glucose in recombinant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42
CPCC12P7/42
Inventor 不公告发明人
Owner XINCHANG GUANYANG TECH DEV
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