A kind of bacteria strain that promotes the germination of dendrobium dendrobium seedlings and its application

A technology of Dendrobium denticola and seeds, which is applied in the field of fungal strains of the genus Meloidea, can solve problems such as being unsuitable for large-scale industrial development, low seedling reproduction coefficient, and low seedling quality, and achieves low cost and non-organized degradation problems. , the effect of easy operation

A technology of Dendrobium denticola and seeds, which is applied in the field of fungal strains of the genus Meloidea, can solve problems such as being unsuitable for large-scale industrial development, low seedling reproduction coefficient, and low seedling quality, and achieves low cost and non-organized degradation problems. , the effect of easy operation

CN104651239BActive Publication Date: 2017-10-13JINGHONG HONGZHEN AGRI TECH CO LTD
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  • A kind of bacteria strain that promotes the germination of dendrobium dendrobium seedlings and its application
  • A kind of bacteria strain that promotes the germination of dendrobium dendrobium seedlings and its application
  • A kind of bacteria strain that promotes the germination of dendrobium dendrobium seedlings and its application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Embodiment I, the isolation and identification of Tulasnella sp.CY bacterial strain

[0025] 1. Isolation of Glycobacteria CY strain

[0026] 1. Acquisition of protocorms: Collect the naturally germinated protocorms of Dendrobium dendrobium near the adult plants of Dendrobium dendrobium tea trees in Longpa ancient tea garden, Jinuo Township, Jinghong City, Xishuangbanna Dai Autonomous Prefecture, and use the moist moss grown on the tea tree trunks Wrapped and brought back to the laboratory for follow-up experiments.

[0027] 2. Induction, separation, purification and preservation of symbiotic fungi in protocorms: Take out the germinated protocorms, put them into a sterilized beaker filled with 1% sodium hypochlorite (NaClO) solution, shake the beaker gently, and use a sterilized blunt tip after 5 minutes Take out the tweezers and rinse with sterile water 3 to 4 times. On the ultra-clean workbench, cut the protocorm in half with a sterile blade, and place it in PDA med...

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Abstract

The invention discloses a strain for promoting the germination of Dendrobium denticola seeds to form seedlings and an application thereof. The strain is classified as Tulasnella sp.CY, and is preserved in the General Microorganism Center of the China Microorganism Culture Collection Management Committee with the deposit number CGMCC NO.9551. The strain can significantly promote the germination of Dendrobium candidum seeds to form seedlings, and shows strong specificity. It is of great practical significance to solve the problem of obtaining symbiotic seedlings through seeds of Dendrobium dendrobii, which can be used for nursery seedlings or live seeding of trunk seeds in the field. Compared with tissue culture, it has the advantages of low cost, high field survival rate, and no tissue degradation. It has the advantages of replacing Tissue culture obtains the application value of symbiotic seedlings, and provides a reliable technical guarantee for solving the bottleneck problem of seedling sources in Dendrobium candidum cultivation industry.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a fungus strain of the genus Colloidal which can effectively promote the symbiotic germination of dendrobium dendrobium seeds to form protocorms, and grow and develop a large number of seedlings. At the same time, the invention also relates to the specific application of the bacterial strain in the breeding of Dendrobium dendrobium. Background technique [0002] As a traditional Chinese medicinal material, Dendrobium devonianum has extremely high medicinal value, and its cultivation scale has developed into the second pillar industry after Dendrobium candidum in some prefectures of Yunnan. There are roughly four sources of seedlings used in the industry: the first is wild plants, which were mainly seen in some small individual enterprises in the 1990s. With the scarcity of wild resources, this practice cannot continue. For the protection of plants, it is ill...

Claims

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Application Information

Patent Timeline
13 Oct 2017
Publication
CN104651239B
IPC
C12N1/14; A01H17/00; C12R1/645
CPC
A01H17/00; C12N1/14; C12N1/145; C12R2001/645; Y02A40/10
Inventors
高江云; 邵士成