Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Wheat anther differentiation culture medium formula

A technology of differentiation medium and culture medium, which is applied in the field of agricultural science, can solve the problems of low efficiency, low induction rate, and small population, and achieve the effects of improving culture efficiency, reducing albino rate, and increasing differentiation rate

Active Publication Date: 2015-06-17
泊头市蔬宝种业有限公司
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of research has been done on wheat anther culture and a flower breeding system has been established, there are still problems of low induction rate, small population, and relatively low efficiency, which cannot meet the needs of breeding, and anther culture is still under research. edge application status

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wheat anther differentiation culture medium formula
  • Wheat anther differentiation culture medium formula
  • Wheat anther differentiation culture medium formula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare the culture medium with the following formula:

[0015] KNO 3 2950mg / L, C 8 h 7 o 2 K 3000mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?H 2 O 3.0mg / L, ZnSO 4 ?H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ?5H 2 O 0.05mg / L, Na 2 MoO 4 ?2H 2 O 0.16mg / L, CoCl 2 ?6H 2 O 0.16mg / L, glycine 2.0mg / L, folic acid 0.8mg / L, methylnitrosourea 2.5mg / L, alanine 5.0mg / L, lysine 2.5mg / L, vitamin B1 5.0mg / L L, vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 30g / L, vegetable gel 5.5g / L;

[0016] NAA 0.8mg / L, KT 1.0mg / L, prohexadione calcium 1.0mg / L, sorbitol 30g / L, hydrolyzed protein 1.5g / L, colchicine 0.03mg / L, biotin 0.16mg / L, PH 5.9.

[0017] The anther calli of wheat varieties Zaoyangmai and Abo were selected as donors. When the anther callus after induction culture grew to 2 mm in size, it was transferred to the differentiation medium t...

Embodiment 2

[0020] The culture medium (C 8 h 7 o 2 The preferred concentration of K added):

[0021] KNO 3 2950mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?H 2 O 3.0mg / L, ZnSO 4 ?H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ?5H 2 O 0.05mg / L, Na 2 MoO 4 ?2H 2 O 0.16mg / L, CoCl 2 ?6H 2O 0.16mg / L, glycine 2.0mg / L, folic acid 0.8mg / L, methylnitrosourea 2.5mg / L, alanine 5.0mg / L, lysine 2.5mg / L, vitamin B1 5.0mg / L L, vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 30g / L, vegetable gel 5.5g / L;

[0022] NAA 0.8mg / L, KT 1.0mg / L, prohexadione calcium 1.0mg / L, sorbitol 30g / L, hydrolyzed protein 1.5g / L, colchicine 0.03mg / L, biotin 0.16mg / L, PH 5.9.

[0023] C 8 h 7 o 2 The added concentration of K is set to the following 8 kinds: 0; 500mg / L; 1000mg / L; 1500mg / L; 2000mg / L; 2500mg / L; 3500mg / L;

[0024] The anther calluses of these two wheat varieties were als...

Embodiment 3

[0026] Prepare the medium with the following formula (preferable concentration of folic acid added):

[0027] KNO 3 2950mg / L, C 8 h 7 o 2 K 3000mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ?7H 2 O 24mg / L, MnSO 4 ?H 2 O 3.0mg / L, ZnSO 4 ?H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ?5H 2 O 0.05mg / L, Na 2 MoO 4 ?2H 2 O 0.16mg / L, CoCl 2 ?6H 2 O 0.16mg / L, Glycine 2.0mg / L, Methylnitrosourea 2.5mg / L, Alanine 5.0mg / L, Lysine 2.5mg / L, Vitamin B1 5.0mg / L, Vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 30g / L, plant gel 5.5g / L;

[0028] NAA 0.8mg / L, KT 1.0mg / L, prohexadione calcium 1.0mg / L, sorbitol 30g / L, hydrolyzed protein 1.5g / L, colchicine 0.03mg / L, biotin 0.16mg / L, PH 5.9.

[0029] The added concentration of folic acid is the following 6 kinds: 0; 0.2mg / L; 0.4mg / L; 0.6mg / L; 1.0mg / L; 1.2mg / L.

[0030] The anther calluses of these two wheat varieties were also sele...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a wheat anther differentiation culture medium formula. The culture medium formula proportionally comprises KNO3, C8H7O2K, (NH4)2SO4, KH2PO4, CaCl2, MgSO4, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, Na2Mo4.2H2O, CoCl2.6H2O, glycine, folic acid, methyl-nitrosourea, alanine, lysine, vitamin B1, vitamin B6, 3-picolinic acid, sucrose, gelling agent, NAA, KT, prohexadione calcium, sorbitol, protein hydrolysate, colchicine and biotin. The culture medium has the advantages of efficiently improving the differentiation rate of the wheat anther callus seedlings and reducing the ablation rate, and the wheat anther culture efficiency can be obviously improved.

Description

technical field [0001] The invention relates to a wheat anther differentiation medium, in particular to a differentiation medium formula for efficiently increasing the differentiation rate of wheat anther callus green seedlings and reducing the albino rate, and belongs to the field of agricultural science and technology. Background technique [0002] Wheat is a gramineous plant widely planted all over the world. It is a general term for Triticum plants and originated in the Fertile Crescent region of the Middle East. Wheat is one of the three major grains, most of which are used as food, and only about one-sixth is used as feed. As the second largest food crop in the world, wheat is also the second largest food crop in China, and plays an important role in the food of Chinese people. According to statistics, wheat accounts for about 43% of the total grain consumption in China. In recent years, the world's population has grown and people's living standards have continued to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 泊头市蔬宝种业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com