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A kind of japonica rice anther differentiation medium formula

A technology for differentiation culture medium and culture medium, applied in the field of agricultural science, can solve the problems of low anther culture ability, low flower culture induction rate, low green seedling differentiation rate, unsatisfactory culture efficiency and the like

Active Publication Date: 2016-07-27
YIELEAD RICE IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Rice flower cultivars have many advantages, but their cultivation efficiency is not very ideal, the main reason is that the induction rate of flower culture and the differentiation rate of green seedlings are low, that is, the anther culture ability is low

Method used

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  • A kind of japonica rice anther differentiation medium formula
  • A kind of japonica rice anther differentiation medium formula
  • A kind of japonica rice anther differentiation medium formula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Prepare the culture medium with the following formula:

[0012] KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, potassium 4-methylbenzoate 2400mg / L, KH 2 PO 4 170mg / L, CaCl 2 ?2H 2 O440mg / L, MgSO 4 ?7H 2 O370mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O27.5mg / L, MnSO 4 ?4H 2 O22.5mg / L, ZnSO 4 ?7H 2 O8.5mg / L, H 3 BO 3 6.2mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, Na 2 MoO 4 ?2H 2 O0.25mg / L, CoCl 2 ?6H 2 O0.025mg / L, inositol 100mg / L, glycine 2.0mg / L, proline 180mg / L, folic acid 0.4mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B10. 4mg / L, vitamin B60.5mg / L, 3-pyridinecarboxylic acid 0.5mg / L, sucrose 25g / L, plant gel 5.5g / L;

[0013] NAA0.5mg / L, KT2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, hydrolyzed protein 1.0g / L, biotin 0.08mg / L, PH5.9.

[0014] The anther calli of japonica rice varieties Tongkejaponica and Heihuzijaponica were selected as donors, and when the induced anther callus grew to 2 mm in size, they were transferred to differentiation medium ...

Embodiment 2

[0016] Prepare the medium with the following formula (the preferred concentration of potassium 4-methylbenzoate added):

[0017] KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, KH 2 PO 4 170mg / L, CaCl 2 ?2H 2 O440mg / L, MgSO 4 ?7H 2 O370mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O27.5mg / L, MnSO 4 ?4H 2 O22.5mg / L, ZnSO 4 ?7H 2 O8.5mg / L, H 3 BO 3 6.2mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, Na 2 MoO 4 ?2H 2 O0.25mg / L, CoCl 2 ?6H 2 O0.025mg / L, inositol 100mg / L, glycine 2.0mg / L, proline 180mg / L, folic acid 0.4mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B10. 4mg / L, vitamin B60.5mg / L, 3-pyridinecarboxylic acid 0.5mg / L, sucrose 25g / L, plant gel 5.5g / L;

[0018] NAA0.5mg / L, KT2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, hydrolyzed protein 1.0g / L, biotin 0.08mg / L, PH5.9.

[0019] The added concentration of potassium 4-methylbenzoate is set in the following 8 types: 0; 400mg / L; 800mg / L; 1200mg / L; 1600mg / L; 2000mg / L; 2800mg / L;

[0020] The anther calli of the two ja...

Embodiment 3

[0022] Prepare the medium with the following formula (preferred concentration of proline added):

[0023] KNO 3 1900mg / L, NH 4 NO 3 1650mg / L, potassium 4-methylbenzoate 2400mg / L, KH 2 PO 4 170mg / L, CaCl 2 ?2H 2 O440mg / L, MgSO 4 ?7H 2 O370mg / L, Na 2 -EDTA37.5mg / L, FeSO 4 ?7H 2 O27.5mg / L, MnSO 4 ?4H 2 O22.5mg / L, ZnSO 4 ?7H 2 O8.5mg / L, H 3 BO 3 6.2mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, Na 2 MoO 4 ?2H 2 O0.25mg / L, CoCl 2 ?6H 2 O0.025mg / L, inositol 100mg / L, glycine 2.0mg / L, folic acid 0.4mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B10.4mg / L, vitamin B60. 5mg / L, 3-pyridinecarboxylic acid 0.5mg / L, sucrose 25g / L, plant gel 5.5g / L;

[0024] NAA0.5mg / L, KT2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, hydrolyzed protein 1.0g / L, biotin 0.08mg / L, PH5.9.

[0025] The added concentration of proline is the following 8 kinds: 0; 30mg / L; 60mg / L; 90mg / L; 120mg / L; 150mg / L; 210mg / L; 240mg / L.

[0026] The anther calli of the two japonica rice varieties were ...

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Abstract

The invention provides a differentiation culture medium for japonica rice anthers. A formula of the culture medium consists of the following ingredients in a certain ratio: KNO3, NH4NO3, C8H7O2K, KH2PO4, CaCl2.2H2O, MgSO4.7H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, Na2MoO4.2H2O, CoCl2.6H2O, inosite, glycine, proline, folic acid, alanine, lysine, vitamin B1, vitamin B6, 3-picolinic acid, saccharose, plant gel, NAA, KT, prohexadione calcium, sorbitol, hydrolyzed protein and biotin. The culture medium provided by the invention has the advantage of efficiently increasing the green-seedling differentiation rate of japonica rice anther caluus and can be used for remarkably increasing the culture efficiency of the japonica rice anthers.

Description

technical field [0001] The invention relates to a japonica rice anther differentiation medium, in particular to a differentiation medium formula for efficiently improving the differentiation rate of japonica rice anther callus and green seedlings, and belongs to the field of agricultural science and technology. Background technique [0002] Anther breeding is a breeding method in which anthers are cultivated into haploid plants, and then chromosomes are naturally or artificially doubled to obtain homozygous diploids, so anther breeding is also called haploid breeding. Rice is one of the most important food crops in the world. In 1968, the cultivation of rice anthers was successful. Subsequently, the use of this technology to breed rice varieties has attracted the attention of breeders from all over the world. The main characteristics of rice anther culture and breeding are shortening the breeding cycle, improving selection efficiency, and accelerating the transfer of effect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 乔保建陶颖程驭胡四宝陶元平
Owner YIELEAD RICE IND
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