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Method for regenerating plants by using hibiscus mutabilis anther calluses

A technology for callus and plant regeneration, which is applied in the field of plant tissue culture, can solve the problems of hibiscus anther culture and regeneration system that have not been seen, and achieve the effect of improving the differentiation rate of green seedlings

Inactive Publication Date: 2020-11-17
LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the research on the culture of hibiscus anthers and the establishment of regeneration system

Method used

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  • Method for regenerating plants by using hibiscus mutabilis anther calluses
  • Method for regenerating plants by using hibiscus mutabilis anther calluses
  • Method for regenerating plants by using hibiscus mutabilis anther calluses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for plant regeneration from hibiscus anther callus, comprising the following steps:

[0025] (1) Differentiation culture of callus: select the anther callus of hibiscus cultivars 'Mudanhong' and 'Zui Furong' as the test material, and transfer the callus with a size of 2 mm or more into the differentiation medium: KNO 3 2045mg / L, NH 4 NO 3 1350mg / L, NaH 2 PO 4 150mg / L, CaCl 2 128mg / L, MgSO 4 ·7H 2 O 395mg / L, MnSO 4 4H 2 O 21.4mg / L, KI0.85mg / L, H 3 BO 3 5.0mg / L, ZnSO 4 ·7H 2 O 3.7mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, CuSO 4 ·5H 2 O0.025mg / L, CoCl 2 ·6H 2 O 0.025mg / L, FeSO 4·7H 2 O 28.3 mg / L, Na 2 -EDTA 37.4mg / L, inositol 90mg / L, niacin 0.8mg / L, folic acid 0.4mg / L, vitamin B1 8.0mg / L, vitamin B6 1.0mg / L, lysine 2.5mg / L, humic Sodium phosphate 4.0mg / L, isopentenyl adenine 2.0mg / L, 6-BA 2.0mg / L, NAA 0.2mg / L, betaine 4.0mg / L, glutamine 205mg / L, γ-aminobutyric acid 5.0mmol / L, nano-titanium dioxide 40mg / L, carrot juice 80ml / L, sucrose 30g / L, agar 7...

Embodiment 2

[0028] Example 2 Effect of sodium humate on callus differentiation of hibiscus anthers

[0029] The anther calli of 'Mudanhong' and 'Zui Furong' were used as the test materials, and they were transferred to the differentiation medium supplemented with different concentrations of sodium humate for cultivation, and the effects of different concentrations of sodium humate on the green effect on seedling differentiation. The concentration of sodium humate was designed to be 0, 1.0, 2.0, 4.0, 8.0mg / L, a total of 5 concentrations, and each concentration was repeated 3 times. The results are shown in Table 1 below.

[0030]

[0031] It can be seen from Table 1 that when 1.0-4.0mg / L sodium humate is added to the medium, the green shoot differentiation rate of the test material also increases with the increase of the concentration of sodium humate, and the addition of 4.0mg / L humate When adding sodium humate, the green seedling differentiation rate is the highest. 'Mudanhong' can ...

Embodiment 3

[0032] Example 3 Effects of nano-titanium dioxide on callus differentiation of hibiscus anthers

[0033] The anther calli of 'Mudanhong' and 'Drunken Hibiscus' were used as test materials, and they were transferred to the differentiation medium added with different concentrations of sodium humate for cultivation, and the effects of different concentrations of nano-titanium dioxide on the green seedling differentiation of the test materials were compared. Impact. The concentration of nano-titanium dioxide was designed to be 0mg / L, 20mg / L, 40mg / L, 60mg / L, 80mg / L, a total of 5 concentrations, and each concentration was repeated 3 times. The results are shown in Table 2 below.

[0034]

[0035] It can be seen from Table 2 that when 20-80mg / L nano-titanium dioxide is added to the medium, the green seedling differentiation rate of the test materials is significantly improved, and the green seedling differentiation rate is the highest when 40mg / L nano-titanium dioxide is added, a...

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PUM

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Abstract

The invention discloses a method for regenerating plants by using hibiscus mutabilis anther calluses. The method comprises the following steps of: transferring calluses with the size of more than 2mmformed by hibiscus mutabilis anther induction culture into a differential medium, culturing under an illumination condition for about 25 days, replacing a new differential medium, and continuing to culture adventitious buds or clustered seedlings which can germinate after 25-35 days; cutting off the adventitious buds or clustered plantlets, transferring the adventitious buds or clustered plantletsto a strong seedling medium, and culturing the adventitious buds or clustered plantlets under the illumination condition for 20-30 days to form complete regenerating plants; and when the regeneratingplants grow into robust seedlings with 4-5 leaves and developed root systems, seedling hardening and transplanting are carried out. By applying the method disclosed by the invention, the regeneratingplants cultured by hibiscus mutabilis anthers are successfully obtained. The proportion of haploid is 9.40% through identification, and a technical basis and an experimental material are provided forfurther developing hibiscus mutabilis haploid breeding and related theoretical researches.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for regenerating plants from hibiscus hibiscus anther callus. Background technique [0002] Hibiscus ( Hibiscus mutabilis L.) Commonly known as hibiscus, frost-resistant flower, magnolia, ground hibiscus, and Chinese wood, it is native to my country and distributed in East Asia and Southeast Asia. Hibiscus hibiscus is a traditional famous flower in my country. It has bright colors, beautiful tree shape, long flowering period and rich colors, including red, pink, white, yellow and other colors. The color of the flower will change with time. There are also "colored hibiscus" that change color every other day, so they have extremely high ornamental value. Hibiscus hibiscus is suitable for growing in a warm and humid environment. It is resistant to water and humidity, but not cold-resistant. It has low requirements on the soil and has strong adapta...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王金鑫金宁佳
Owner LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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