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A kind of japonica rice anther differentiation medium formula

A technology of differentiation medium and culture medium, which is applied in the field of agricultural science, and can solve the problems of small regenerated plant population, limited selection and utilization, and insufficient phenotype of offspring after flower culture

Active Publication Date: 2016-12-14
宝应中宝德园有机农庄
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the influence of medium components, differences in materials, and cultivation environment, the current flower cultivation efficiency is not ideal, the regenerated plant population obtained is small, and the phenotypes of the flower culture offspring are not sufficient, which makes selection and utilization more difficult. limits

Method used

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  • A kind of japonica rice anther differentiation medium formula
  • A kind of japonica rice anther differentiation medium formula
  • A kind of japonica rice anther differentiation medium formula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Prepare the culture medium with the following formula:

[0013] KNO 3 2950mg / L, C 8 h 7 o 2 K 1900mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ∙7H 2 O 24mg / L, MnSO 4 ∙H 2 O 3.0mg / L, ZnSO 4 ∙H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ∙5H 2 O 0.05mg / L, Na 2 MoO 4 ∙2H 2 O0.16mg / L, CoCl 2 ∙6H 2 O 0.16mg / L, glycerol 0.9mg / L, glycine 2.0mg / L, folic acid 0.4mg / L, methylnitrosourea 1.2mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B1 5.0mg / L, vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 25g / L, plant gel 5.5g / L;

[0014] NAA 0.05mg / L, 6-BA 2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, L-proline 120mg / L, hydrolyzed protein 1.0g / L, colchicine 0.2mg / L, biotin 0.08mg / L, pH 5.9.

[0015] The anther calli of japonica rice varieties Tongkejaponica and Heihuzijaponica were selected as donors, and when the induced anther callus grew to 2 mm in size, they ...

Embodiment 2

[0018] The culture medium (C 8 h 7 o 2 The preferred concentration of K added):

[0019] KNO 3 2950mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ∙7H 2 O 24mg / L, MnSO 4 ∙ H 2 O 3.0mg / L, ZnSO 4 ∙ H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ∙5H 2 O 0.05mg / L, Na 2 MoO 4 ∙2H 2 O 0.16mg / L, CoCl 2 ∙6H 2O0.16mg / L, glycerin 0.9mg / L, glycine 2.0mg / L, folic acid 0.4mg / L, methylnitrosourea 1.2mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B1 5.0mg / L, vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 25g / L, plant gel 5.5g / L;

[0020] NAA 0.05mg / L, 6-BA 2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, L-proline 120mg / L, hydrolyzed protein 1.0g / L, colchicine 0.2mg / L, biotin 0.08mg / L, pH 5.9.

[0021] C 8 h 7 o 2 The addition concentration of K is set in the following 8 types: 900mg / L; 1100mg / L; 1300mg / L; 1500mg / L; 1700mg / L; 2100mg / L; 2300mg / L;

[0022] The anther c...

Embodiment 3

[0024] Prepare the medium with the following formula (the preferred concentration of glycerol added):

[0025] KNO 3 2950mg / L, C 8 h 7 o 2 K 1900mg / L, (NH 4 ) 2 SO 4 430mg / L, KH 2 PO 4 350mg / L, CaCl 2 110mg / L, MgSO 4 85mg / L, Na 2 -EDTA 32.5mg / L, FeSO 4 ∙7H 2 O 24mg / L, MnSO 4 ∙ H 2 O 3.0mg / L, ZnSO 4 ∙ H 2 O 1.3mg / L, H 3 BO 3 1.4mg / L, KI 0.65mg / L, CuSO 4 ∙5H 2 O 0.05mg / L, Na 2 MoO 4 ∙2H 2 O0.16mg / L, CoCl 2 ∙6H 2 O 0.16mg / L, glycine 2.0mg / L, folic acid 0.4mg / L, methylnitrosourea 1.2mg / L, alanine 1.2mg / L, lysine 1.4mg / L, vitamin B1 5.0mg / L L, vitamin B6 1.0mg / L, 3-pyridinecarboxylic acid 1.0mg / L, sucrose 25g / L, vegetable gel 5.5g / L;

[0026] NAA 0.05mg / L, 6-BA 2.0mg / L, prohexadione calcium 0.7mg / L, sorbitol 25g / L, L-proline 120mg / L, hydrolyzed protein 1.0g / L, colchicine 0.2mg / L, biotin 0.08mg / L, pH 5.9.

[0027] The added concentration of glycerol is the following 6 kinds: 0; 0.3mg / L; 0.6mg / L; 1.2mg / L; 1.5mg / L; 1.8mg / L.

[0028] The anther calli ...

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Abstract

The invention provides a japonica rice anther differential culture medium formula. According to the formula, a culture medium is composed of KNO3, C8H7O2K, (NH4)2SO4, KH2PO4, CaCl2, MgSO4, Na2-EDTA, FeSO4.7H2O, MnSO4.H2O, ZnSO4.H2O, H3BO3, KI, CuSO4.5H2O, Na2MoO4.2H2O, CoCl2.6H2O, glycerol, glycine, folic acid, methyl-nitrosourea, alanine, lysine, vitamin B1, vitamin B6, 3-picolinic acid, saccharose, plant gel, NAA, 6-BA, prohexadione calcium, sorbitol, L-proline, protein hydrolysate, colchicine and biotin. The culture medium has the advantages of being effectively increasing the healing green plantlet differentiation rate of japonica rice anther and reducing the albation rate, and the culture efficiency of the japonica rice anther can be obviously improved.

Description

technical field [0001] The invention relates to a japonica rice anther differentiation medium, in particular to a differentiation medium formula for efficiently increasing the differentiation rate of japonica rice anther callus green shoots and reducing the albino rate, and belongs to the field of agricultural science and technology. Background technique [0002] Anther culture is the use of plant tissue culture technology to inoculate anthers that have developed to a certain stage on an artificial medium through aseptic techniques to change the developmental program of pollen grains in anthers, induce their differentiation, and continuously undergo mitosis to form cells. Group, and then form a group of undifferentiated parenchyma-callus, or differentiate into embryoid bodies, and then make the callus differentiate into a complete plant, so anther breeding is also called haploid breeding. [0003] Rice is one of the most important food crops in the world. In 1968, Japan's S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 许伟
Owner 宝应中宝德园有机农庄
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