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Signal transduction and transcriptional activation factor inhibitory polypeptide and application thereof

A technique of transcriptional activator and inhibitory polypeptide, which is applied in the field of relapsed and metastatic polypeptides to improve the survival rate

Inactive Publication Date: 2015-06-24
SUZHOU PULUODA BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no well-developed signal transduction and transcription activator inhibitory polypeptide, which is used to prevent and treat cancer recurrence and metastasis after breast cancer surgery

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Signal transduction and transcription activator inhibit the migration of polypeptide breast cancer cells MCF-7.

[0015] Use a scratch test. First use a marker pen on the back of the 24-well plate, compare it with a straightedge, and draw evenly horizontal lines, about every 0.5~1cm, across the holes. Each hole passes through 3 lines; the logarithmic growth of MCF-7 cells is divided into 1.0×10 5 Add it to a 24-well culture plate and incubate for 24h. On the second day, use a 10μl pipette tip to compare with a ruler, and make a scratch perpendicular to the horizontal line on the back. The intersection of the scratch and the horizontal line on the back is the fixed observation site; the experiment hole and the positive drug control hole are respectively added with different concentrations of experiment Drug signal transduction and transcription activator inhibitor peptide (Shanghai Shenggong Synthetic) and positive control drug vincristine; the blank group is added with th...

Embodiment 2

[0017] The signal transduction and transcription activator inhibitor polypeptide has an IC50 for the growth and survival of cultured human breast cancer cells in vitro.

[0018] Using MTT colorimetric method. The logarithmic growth of human breast cancer cells was calculated as 1.0×10 5 Add to 96-well culture plate, culture for 24 hours, add different concentrations of experimental drug signal transduction and transcription activator inhibitor peptide (Shanghai Shenggong Synthetic) and positive control drug paclitaxel to the experimental wells and positive drug control wells; add the same volume of the blank group Solvent. Set up five multiple wells in each well, culture for 48h, respectively at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h. Add MTT to each well. After 4h, add DMSO and incubate for 30min. Determine the absorbance A value, according to the formula human breast cancer cell growth inhibition rate = (1-experimental group absorbance value / control group absorbance value) ...

Embodiment 3

[0020] Use tumor models to detect the in vivo activity of signal transduction and transcription activator inhibitors.

[0021] The MCF-7 tumor model was established, the positive control drug paclitaxel; the blank group was added with the same volume of solvent, and the experimental group peptide (Shanghai Shenggong Synthesis) was set at 3 doses: 0.75, 1.5, 3μM mg / Kg. After 21 days, observe the number of surviving mice and calculate the survival rate. The results show that the signal transduction and transcription activator inhibitor polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice, with a survival rate of 61.5%.

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PUM

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Abstract

The invention relates to the field of medicines, and in particular relates to a polypeptide provided with an inhibiting signal transduction and transcriptional activation factor and used for preventing and treating cancer recurrence and metastasis after breast cancer. The sequence of the polypeptide is MEQFRHLRANPSWPHLQMFP and is a brand-new sequence, the sequence can be used for in vitro inhibiting proliferation and migration of breast cancer cell, the survival rate of the tumor-bearing mice is improved in an in vivo test, and the polypeptide has potential new medicine development value.

Description

technical field [0001] The present invention relates to signal transduction and transcription activator inhibitory polypeptide and application thereof, in particular to a polypeptide capable of inhibiting signal transduction and transcription activator to prevent and treat breast cancer recurrence and metastasis after breast cancer surgery. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. At present, breast cancer has become a common tumor that threatens women's physical and mental health. [0003] The mammary gland is not an important organ to maintain human life activities, and breast cancer in situ is not fatal; but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once the cancer c...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P35/00A61P35/04
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
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