Signal transduction and activating transcription factor inhibiting polypeptide
A technique of transcriptional activator and inhibitory polypeptide, which is applied in the field of relapsed and metastatic polypeptides to improve the survival rate
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Embodiment 1
[0010] Signal Transducer and Transcription Activator Inhibiting the Migration of Peptide Breast Cancer Cell MCF-7.
[0011] Using scratch test. First use a marker pen on the back of the 24-well plate, draw a horizontal line evenly with a straightedge, about every 0.5-1cm, across the hole. Pass 3 lines per well; MCF-7 cells grown in logarithmic 5 Added to 24-well culture plate, cultivated for 24h. On the second day, compare the ruler with a 10 μl pipette tip, scratch perpendicular to the horizontal line on the back, and use the intersection of the scratch and the horizontal line on the back as a fixed observation point; add different concentrations of experimental wells and positive drug control wells respectively. Drug signal transduction and transcription activator inhibitory polypeptide (Shanghai Sangong Synthetic) and positive control drug vincristine; the same volume of solvent was added to the blank group, and five duplicate holes were set in each hole; put in 37 ° C, 5...
Embodiment 2
[0013] Signal Transduction and Transcription Activator Inhibiting Polypeptide IC50 on Growth and Survival of Human Breast Cancer Cells Cultured in Vitro.
[0014] The MTT colorimetric method was used. Human breast cancer cells growing logarithmically at 1.0×10 5 Added to 96-well culture plate, cultured for 24h, experimental wells, positive drug control wells were added different concentrations of experimental drug signal transduction and transcription activator inhibitory polypeptide (Shanghai Sangong Synthetic) and positive control drug paclitaxel; the blank group was added the same volume of solvent. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and incubate at 620nm in a microplate reader The absorbance A value was measured, according to the formula human breast cancer cell growth inhibition rate=(1-absorbance value of the experimental gr...
Embodiment 3
[0016] The in vivo activity of the signal transduction and transcriptional activator inhibitory polypeptide was tested in a tumor model.
[0017] The MCF-7 tumor model was established, and the positive control drug paclitaxel was added; the same volume of solvent was added to the blank group, and three doses of polypeptide (Shanghai Sangong Synthetic) were set in the experimental group: 0.75, 1.5, and 3 μM mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results show that the signal transduction and transcription activator inhibitory polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice, with the survival rate reaching 49.2%.
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