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Application of pectolinarin in preparation of anti-osteosarcoma medicine and anti-osteosarcoma medicine preparation

A technology of leaf glycosides and pharmaceutical preparations of chrysalis, which is applied in the field of medicine, can solve problems such as adverse reactions and large toxic and side effects of western medicines, achieve the effects of inhibiting proliferation, solving large toxic and side effects, and improving the therapeutic effect

Pending Publication Date: 2022-01-11
LUOYANG ORTHOPEDIC TRAUMATOLOGICAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide the application and anti-osteosarcoma drug preparations of Salmonella japonica leaf glycosides in the preparation of anti-osteosarcoma drugs, so as to solve the problems in the above-mentioned prior art, thereby solving the toxic and side effects of existing western medicines for osteosarcoma treatment Large, serious adverse reaction problems

Method used

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  • Application of pectolinarin in preparation of anti-osteosarcoma medicine and anti-osteosarcoma medicine preparation
  • Application of pectolinarin in preparation of anti-osteosarcoma medicine and anti-osteosarcoma medicine preparation
  • Application of pectolinarin in preparation of anti-osteosarcoma medicine and anti-osteosarcoma medicine preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Effects of Lemonfish leaf glycosides on cell proliferation

[0042] (1) MTT method was used to detect the effects of lobster leaf glycosides on the proliferation of 143B, MG-63, U2OS, Sao-2 and HOS cells.

[0043] Cells were incubated at 37°C, 5% CO 2 In the incubator, the MEM medium containing 10% fetal bovine serum was used for monolayer subculture in a 10mm culture dish, and the cells in the logarithmic growth phase were taken for experiments. Cell digestion and counting, 8000 cells per well were seeded in a 96-well plate, and adhered to the wall for 24 hours. The leaf glycosides of willowfish were dissolved in DMSO to make 50μmol·mL -1 Stock solution, diluted to 10, 20, 40, 80, 160 μM, 320 μM with MEM. Each group was added different concentrations of salivary carp leaf glycosides (10, 20, 40, 80, 160, 320 μ M), and each concentration was parallel to 6 wells. In the blank control group, an equal volume of MEM medium containing 10% fetal bovine serum was ...

Embodiment 2

[0048] Example 2 Salmonella leaf glycoside inhibits the clone formation ability of human osteosarcoma cell 143B, HOS

[0049] The 143B and HOS cells in the logarithmic growth phase were digested and counted, about 1000 cells per well were seeded in a 12-well plate, and grown adherently for 24 hours. The cells were intervened by the addition of lobster leaf glycoside at the final concentration of 50, 100, and 150 μM, and a blank control group without lobster leaf glycoside was set up. After acting for 14 days, the cells in the 12-well plate were stained with crystal violet solution, and the formation of cell colonies in each group was photographed.

[0050] figure 2 The cell proliferation results and statistical histograms of the colony formation experiment of the effect of different concentrations of salivary carp leaf glycosides on the proliferation ability of osteosarcoma cells; among them, Figure A is the result of the clone formation test of the cell proliferation, and F...

Embodiment 3

[0052] Example 3 Annexin V-FITC / PI double-staining to detect the effect of the leaf glycosides of Salix carina on the apoptosis rate

[0053] Annexin V-FITC / PI Cell Apoptosis Detection Kit (KGI, China) was used to detect the effect of different doses of fenugreek leaf glycosides intervened for 48 hours on the apoptosis rate of 143B and HOS cells. The kit includes Binding buffer, AnnexinV-FITC and Propidiμm Iodide staining solution.

[0054] 143B and HOS cells digested and counted in the logarithmic growth phase, 10 per well 6 Cells were seeded in 6-well plates and grown for 24 h. The final concentrations of 50, 100, and 150 μM lobster leaf glycosides were added to act for 48 hours, and a blank control group (control) without lobster leaf glycosides was established at the same time. Collect the supernatant and cells, centrifuge at 1000rpm for 5min, remove the medium, wash twice with PBS, and resuspend the cells in 500μL Binding buffer to make the cell concentration 2×10 6 in...

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Abstract

The invention discloses application of pectolinarin in preparation of an anti-osteosarcoma medicine and an anti-osteosarcoma medicine preparation, and belongs to the technical field of medicines. In-vivo and in-vitro experiments prove that the pectolinarin can inhibit growth and proliferation of various osteosarcoma cells, can promote apoptosis of the osteosarcoma cells, can intervene the cell cycle, can retard the cells in the G1 stage, can reduce the number of the cells in the G2 stage and can inhibit the cell division ability; the invasion and migration of HOS and 143B osteosarcoma cells can be inhibited; and animal experiments prove that pectolinarin can obviously inhibit the volume of nude mouse subcutaneous 143B cytoma and can well inhibit the growth of osteosarcoma. The pectolinarin is expected to become a medicine for treating osteosarcoma and has a wide prospect in the aspect of development of the medicine for treating osteosarcoma.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to the application of the leaf glycosides of salivary fish in the preparation of anti-osteosarcoma drugs and anti-osteosarcoma drug preparations. Background technique [0002] Osteosarcoma is the most common primary bone tumor, mostly occurring in adolescents, with an annual incidence of 2-5 / 1 million people. Osteosarcoma mainly manifests as local invasion and early lung metastasis, with a high recurrence rate and extremely poor prognosis, so it is very likely to cause death or disability of patients. At present, osteosarcoma mainly adopts surgical treatment, adjuvant chemotherapy, radiotherapy, targeted and immunotherapy. However, the high heterogeneity of osteosarcoma leads to its resistance to the current conventional chemotherapy drugs, coupled with the great side effects of conventional chemotherapy drugs such as methotrexate, doxorubicin, cisplatin, and ifosfamide It affec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K36/28A61P35/00
CPCA61K31/7048A61K36/28A61P35/00
Inventor 马琨张川王丹丹申晟
Owner LUOYANG ORTHOPEDIC TRAUMATOLOGICAL HOSPITAL
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