Method for establishing mouse simulative transportation stress model

A technology for transport stress and method establishment, applied in the direction of animal husbandry, etc., can solve the problems of increased expenses for experimental animals, and achieve the effects of stable and reliable characteristics, low cost, and increased expenses

Inactive Publication Date: 2015-07-01
HEBEI UNIV OF ENG
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  • Abstract
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Problems solved by technology

In addition, the cost of experimental animal fees will increase significantly

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  • Method for establishing mouse simulative transportation stress model
  • Method for establishing mouse simulative transportation stress model
  • Method for establishing mouse simulative transportation stress model

Examples

Experimental program
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Embodiment Construction

[0022] The present invention will be described in detail below in conjunction with examples.

[0023] Experiment 1: Observation on the influence of transportation stress on mouse blood glucose, serum adrenal cortex hormone, and lactate dehydrogenase:

[0024] 50 Km mice (half female, half male, body weight between 18-22g) were randomly divided into two groups and placed in mouse cages respectively. Shake a group of mice continuously for 8 hours on a shaker at 170 beats / min at room temperature (22-25°C). After the shaking interval of 1 hour, 3 mice in each group were decapitated to collect blood, and left for 30 minutes. After the blood samples were coagulated, they were centrifuged at 3000 rpm / min for 5 minutes. ELISAL kits were used to detect the levels of blood glucose, adrenal cortex hormone and serum lactate dehydrogenase.

[0025] test results:

[0026] The test results of blood glucose, serum adrenal cortex hormone and lactate dehydrogenase were as follows: figure 1 ...

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Abstract

The invention discloses a method for establishing a mouse simulative transportation stress model. Stress time determination and verification testing are conducted through shaking table simulative transportation stress stimulation. The transportation stress model is low in cost, stable and reliable in feature, and capable of effectively solving the problem that the consistency of transportation stress conditions cannot be guaranteed easily when big animals such as cows and pigs are used for establishing the stress model. The bigger the experimental animals are, the more obvious the uncertainty of the influence of factors on experimental results, the transportation stress stimulation factors comprise experimental environmental conditions, self factors of experimental animals, transportation stress stimulation factors and animal fixation factors, and the needed costs of the experimental animals can be increased obviously. The method for the established simulative transportation stress model which is low in cost and stable and reliable in feature can promote the research on transportation stress mechanisms and intervening measures.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for establishing a mouse simulated transportation stress model. Background technique [0002] Under most stress conditions, the biological functions of the animal body will be damaged to varying degrees, such as growth retardation, productivity and fecundity decline, which is the price the body must pay to ensure its basic life activities. When an animal is under stress, if the inherent reserves in the body are not enough to cope with the stress, the body will use the resources that should be used for other biological functions, which will lead to the damage of these biological functions. Under the comprehensive effects of transportation stress sources such as hunger, thirst, mixing, crowding, high temperature, turbulence, and noise during transportation, the nutrition and water consumption of cattle, pigs and other animals are greatly consumed and immunity is reduced, which n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 石玉祥安永福索慧娜王晓芳李英栾英凡王艳梅
Owner HEBEI UNIV OF ENG
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