Method for extracting bilirubin from ox bile by using fermentation process

Abstract

The invention provides a method for extracting bilirubin from ox bile by using a fermentation process. The method mainly comprises the following four steps: fermenting ox bile, sterilizing an ox bile fermentation solution, forming bilirubin, extracting and refining bilirubin. By virtue of the method, high-yield beta-glucuronidase and ox-bile-resistant clostridium perfringens are added into the animal ox bile and fermented; a mercaptoalcohol reducing agent is added, so that the effects of improving the activity of beta-glucuronidase and ox-bile-resistant clostridium perfringens and resisting oxidants are implemented; the overall process is greatly optimized under the control of the special condition; the production efficiency is improved; the cost is reduced; the yield of the finally prepared bilirubin is 0.010-0.014%; and the purify of the bilirubin reaches over 92%.

Description

technical field

[0001] The invention relates to a method for extracting bilirubin, in particular to a method for extracting bilirubin from bovine bile by using a fermentation method. Background technique

[0002] Bilirubin has a variety of pharmacological effects and is the main raw material for the manufacture of artificial bezoar. It is widely used in medicine and is in great demand in the market. However, the current extraction methods of bilirubin are mainly extracted from various animal bile (pig, cattle, sheep), and the content of bilirubin in animal bile is extremely low, the extraction process is cumbersome, and the extraction rate is low (such as Wu The yield of the product obtained by Weiping and others by the direct and rapid extraction method is 0.008%-0.013%, the yield obtained by Shang Wenxue and others by the resin method is 0.006174%, and the yield of the cholecalcium salt method is lower and the production cost is high, resulting in Bilirubin is extremely e...