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Method for enhancing immunological performance and migration capacity of adipose mesenchymal stem cell

A mesenchymal stem cell and immunology technology, applied in the field of enhancing the immune regulation function and migration ability of adipose-derived mesenchymal stem cells, can solve the problems of patient tolerance and adverse reactions, achieve tissue repair and immune regulation function, good effect, and improve The effect of migratory ability

Inactive Publication Date: 2015-08-19
DALIAN UNIV OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

The traditional treatment options for autoimmune diseases are mostly based on glucocorticoids and immunosuppressants. Although they can effectively suppress abnormal autoimmunity, long-term use will cause patient tolerance and many adverse reactions

Method used

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  • Method for enhancing immunological performance and migration capacity of adipose mesenchymal stem cell
  • Method for enhancing immunological performance and migration capacity of adipose mesenchymal stem cell
  • Method for enhancing immunological performance and migration capacity of adipose mesenchymal stem cell

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Experimental program
Comparison scheme
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Embodiment Construction

[0037] (1) Isolation and primary culture of adipose-derived mesenchymal stem cells

[0038] ① 6-week-old C57BL / 6J male mice were anesthetized with ether to kill them; the whole body was disinfected with alcohol;

[0039] ②The white adipose tissue in the groin was cut under sterile conditions, put into HBSS buffer containing double antibodies (penicillin + streptomycin), and washed repeatedly to remove blood, hair and other sundries. Then wash twice with PBS buffer containing double antibody.

[0040] ③ Fully shred the adipose tissue with sterilized surgical scissors, add 2 times the volume of 0.1% type I collagenase, and digest at 37°C for 4 hours with constant temperature and shaking.

[0041] ④ Filter the digested suspension with a nylon cell mesh with a pore size of 70 μm to filter out the tissue suspension, transfer the filtrate to a 15 mL centrifuge tube, and centrifuge at 500 rcf for 10 minutes at room temperature.

[0042] ⑤ After the centrifugation is completed, suck...

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Abstract

The invention relates to the technical field of adipose mesenchymal stem cells in the technical field of cell therapy, in particular to a method for enhancing an immune regulating function and a migration capacity of adipose mesenchymal stem cells. Primary culture and secondary culture are performed to the adipose mesenchymal stem cells obtained through separation, and then short-time culture is performed by using culture medium containing low-concentration TLR3 activator Poly(I:C). After Poly(I:C) co-stimulation culture is performed, the obtained adipose mesenchymal stem cells with a brand new phenotype have a stronger migration capacity, the levels of secreting cytokine and chemotactic factor are changed and a stronger immunosuppressive function is reflected on the whole.

Description

technical field [0001] The present invention relates to the technical field of cell therapy technology field of adipose-derived mesenchymal stem cells, in particular to a method for enhancing the immune regulation function and migration ability of adipose-derived mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs), as a type of adult stem cells, have been extensively studied in the fields of immunotherapy and regenerative medicine due to their unique biological characteristics. This fibroblast-like cell was first discovered in 1968, and it was confirmed in subsequent studies that MSCs can be isolated from various tissues and can be cultured and expanded in vitro. [0003] MSCs are currently widely used in the fields of tissue engineering, injury repair, and autoimmune diseases, due to the following characteristics of MSCs: [0004] 1) Able to migrate to tissue damage sites in vivo; [0005] 2) Possess multidirectional differentiation potenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 张秀娟张凯哲贺高红尹文娟吴倩倩齐延凯
Owner DALIAN UNIV OF TECH
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