A method for extracting succinic acid using succinic acid fermentation broth
An extraction method, succinic acid technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of large sewage discharge, waste of auxiliary materials, high recycling costs, etc., and achieve the effect of reducing the difficulty of the process
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[0039] A method for extracting succinic acid using a succinic acid fermented liquid, the extraction method is as follows:
[0040] (1) Preparation of succinic acid fermentation broth
[0041] ① Weigh 5 kg of peptone, 20 kg of corn steep liquor, 20 kg of glucose, 1.5 kg of potassium dihydrogen phosphate, 1.5 kg of disodium hydrogen phosphate, 5 kg of yeast extract, and 947 kg of deionized water to form a seed medium with a total mass of 1000 kg, and put it in a fermentation kettle Use seed culture medium to cultivate Actinobacillus succinic acid, and the per minute volume intake of carbon dioxide is 10L in the fermenter, and the flow of magnesium carbonate mass concentration in the process is 20% magnesium carbonate suspension to maintain the pH of the system The value is stable at 6.0-7.0;
[0042] ② Weigh 50 kg of peptone, 100 kg of corn steep liquor, 1000 kg of glucose, 25 kg of potassium dihydrogen phosphate, 25 kg of disodium hydrogen phosphate, 50 kg of yeast extract, 50...
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