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Method for extracting active substances from natural plants

A technology of active substances and natural plants, applied in the field of extracting active substances

Inactive Publication Date: 2015-09-23
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the above situation, in order to solve the defects of the prior art, the object of the present invention is to provide a method for extracting active substances from natural plants, which can effectively solve the problem of tumor treatment

Method used

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  • Method for extracting active substances from natural plants
  • Method for extracting active substances from natural plants
  • Method for extracting active substances from natural plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Crushing and extraction of raw materials: dry the leaves of Zanthoxylum bungeanum in the sun, crush them into coarse powder, pass through a 20-mesh sieve, put them in a container, and use 60% ethanol with a mass concentration of 1 times the weight of Zanthoxylum bungeanum powder Soak for 4 hours, put it into a percolation cylinder, use 10 times the weight of the Zanthoxylum bungeanum pepper powder to carry out percolation extraction with aqueous ethanol with a mass concentration of 60%, and collect the percolation extract at a flow rate of 7 mL / min;

[0016] 2) Flash concentration: flash concentration of the obtained extract to 1 / 10 of the original volume by using a vacuum film concentration device below 70°C;

[0017] 3) Decolorization and impurity removal: disperse the concentrated solution in water, the amount of water is one-third of the volume of the concentrated solution, and extract twice with petroleum ether, each time the amount is twice the volume of the con...

Embodiment 2

[0023] 1) Grinding and extraction of raw materials: dry the leaves of Zanthoxylum bungeanum in the sun, grind them into coarse powder, pass through a 20-mesh sieve, put them in a container, and use 70% ethanol with a mass concentration of 1 times the weight of Zanthoxylum bungeanum powder Soak for 5 hours, put it into a percolation cylinder, use 70% aqueous ethanol with a mass concentration of 12 times the weight of the Zanthoxylum bungeanum powder for percolation extraction, and collect the percolation extract at a flow rate of 6 mL / min;

[0024] 2) Flash concentration: The obtained extract is flash-concentrated to 1 / 12 of the original volume by using a vacuum film concentration device below 70°C to obtain a concentrate;

[0025] 3) Decolorization and impurity removal: disperse the concentrated solution in water, the amount of water is one-third of the volume of the concentrated solution, and extract 4 times with petroleum ether, each time the amount is 1.5 times the volume of...

Embodiment 3

[0031] 1) Crushing and extraction of raw materials: dry the leaves of Zanthoxylum bungeanum in the sun, crush them into coarse powder, pass through a 40-mesh sieve, put them in a container, and use 80% ethanol with a mass concentration of 1 times the weight of Zanthoxylum bungeanum powder Soak for 6 hours, put it into a percolation cylinder, use 80% ethanol with a mass concentration of 13 times the weight of the Zanthoxylum bungeanum powder for percolation extraction, and collect the percolation extract at a flow rate of 8 mL / min;

[0032] 2) Flash concentration: The obtained extract is flash concentrated to 1 / 13 of the original volume by using a vacuum film concentration device below 70°C;

[0033] 3) Decolorization and impurity removal: disperse the concentrated solution in water, the amount of water is one-third of the volume of the concentrated solution, and extract 5 times with petroleum ether, each time the amount is 1 times the volume of the concentrated solution, to rem...

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Abstract

The invention relates to a method for extracting active substances from natural plants. By adoption of the method, the problem of tumor treatment can be effectively solved. The method adopting the technical scheme comprises the following steps: (1) crushing and extracting materials; (2) carrying out flash evaporation and concentration; (3) decoloring and removing impurities; (4) preparing a diethyl-ether extraction part; (5) preparing an ethyl-acetate extraction part; (6) preparing a normal-butanol extraction part; and (7) preparing the active substance. The method has the advantages that the active substances extracted from zanthoxylum avicennae and are used for preparing a medicine for treating the tumor, so that the problem of prevention and treatment of the tumor is solved, a new purpose of the zanthoxylum avicennae for treating the tumor is opened up and a great innovation is realized on the traditional Chinese medicine.

Description

technical field [0001] The invention relates to the field of natural plant extraction, in particular to a method for extracting active substances from natural plants. Background technique [0002] Zanthoxylum avicennae is a plant of the genus Zanthoxylum avicennae in the Rutaceae family. It is mainly distributed in areas south of about 25° north latitude, and grows in low-altitude flats, slopes or valleys. my country is distributed in southern regions such as Hainan, Fujian, Guangdong, Taiwan, Guangxi, and Yunnan. In Sanya, Hainan, the resources of Zanthoxylum bungeanum are abundant. It is reported in the literature that the Rutaceae Zanthoxylum plant mainly contains volatile oil, flavonoids, alkaloids, coumarin, amides and other components. The fresh leaves, root bark and pericarp all have the smell of prickly ash, sticky when chewed, bitter and numb tongue, and the pericarp and root bark have a strong smell. Folk used as herbal medicine. It has the effects of expelling...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/758A61P35/00
Inventor 李玉贤崔红新程方荣邓得英王玲
Owner HENAN UNIV OF CHINESE MEDICINE
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