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Root wetting method of high-concentration IAA gel

A high-concentration, gel technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, instability, etc., and achieve the effect of reducing water evaporation and easy operation.

Inactive Publication Date: 2015-10-28
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for dipping roots of high-concentration IAA gel, characterized in that the method includes hormone mother liquor preparation, working solution preparation, hormone gel preparation, root dipping, cuttings, and the specific steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:2 into a high-concentration hormone working solution with a concentration of 500mg / L;

[0016] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone working solution with a concentration of 500mg / L in a water bath at 50°C for later use;

[0017] Root dipping: Dip the lower end of the 8-10cm long, healthy and strong boxwood bran...

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PUM

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Abstract

The invention discloses a root wetting method of a high-concentration IAA gel, belonging to the technical field of plant biology. Firstly IAA mother solution of 1mg / mL is prepared; the mother solution is diluted into high-concentration IAA working solution of 500 mg / L; by using the working solution, high-concentration IAA gel confining liquid of sodium carboxymethylcellulose with the mass concentration of 1 percent and sodium alginate with the mass concentration of 8 percent is prepared; the lower end of a healthy, thick and solid branch is wetted in the gel confining liquid for 3 to 5 seconds, and then is taken out; the branch is inserted into a culture medium for rooting.

Description

technical field [0001] The invention discloses a method for dipping roots with high-concentration IAA gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G7/06A01G1/00
Inventor 钱夕刚
Owner 钱夕刚