A kind of chromium chelated immune complex and its preparation method and application

An immune complex and chelating technology, applied in the medical field, can solve the problems of changes in the content of circulating immune complexes, changes in function, and increased susceptibility to diseases.

Inactive Publication Date: 2017-12-15
广州锦海骢健康科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But how does environmental pollution stimulate the body to significantly increase the CIC content? Is it caused by one or multiple pollutants? Whether the increase in CIC will cause damage to the body and increase the susceptibility of the body to diseases? all to be studied
As we all know, circulating immune complexes are proteins of special significance, and chromium can act on proteins in multiple systems throughout the body. Whether chromium can act on circulating immune complexes will change the content, activity and function of circulating immune complexes , there is still a lack of relevant research on these

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of chromium chelated immune complex and its preparation method and application
  • A kind of chromium chelated immune complex and its preparation method and application
  • A kind of chromium chelated immune complex and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0062] Wherein, the preparation method includes:

[0063] A) the step of synthesizing chromium chelating type immune complex;

[0064] B) The step of purifying the chromium-chelating immune complex: using immunoaffinity chromatography to remove the carrier protein, specific antibody and chromium ions in the chromium-chelating immune complex synthesized in step A) that do not participate in the reaction.

[0065] Specifically, in the preparation method of the chromium chelated immune complex,

[0066] Described step A) concrete steps are as follows:

[0067] (1) prepare chelating agent: chelating agent is dissolved in solvent, is mixed with chelating agent solution;

[0068] (2) Prepare the carrier protein solution: add the carrier protein to the borate solution;

[0069] (3) Stirring overnight: adding the chelating agent solution in step (1) to the carrier protein solution in step (2), stirring and acting in a shaker for 2-30 hours to obtain a mixed solution;

[0070] (4) ...

Embodiment 1

[0163] The reagents used in this embodiment are as follows:

[0164] Borate buffer solution, its molar concentration is 0.01M, and this borate buffer solution of every 500ml is made as follows again: take by weighing 0.31g boric acid and be dissolved in 400ml ultrapure water, adjust pH to with the NaOH aqueous solution of 0.1M 9.0, set the volume to 500mL, that is;

[0165] Human serum albumin solution, its preparation method is as follows: take human serum albumin and dissolve it in the above-mentioned borate buffer solution with molar concentration of 0.01M and pH of 9.0, shake and dissolve fully, and prepare human serum albumin of 1.0mg / mL solution;

[0166] 5mmol / L EDTA+200mmol / LNaHCO 3 Solution, 5mmol / L EDTA+200mmol / LNaHCO per 1000ml 3 The solution is prepared by the following method: Take 1.86g EDTA 2H 2 O, 16.8g NaHCO 3 , add 900mL of ultrapure water, then adjust the pH to 8.0 with a NaOH aqueous solution with a molar concentration of 1.0M, then add ultrapure water...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Dilution degreeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a chromium chelating type immune complex as well as a preparation method and application thereof. The chromium chelating type immune complex is one kind of the following complexes of: a complex formed by combination of a chromium ion and the immune complex, or a complex formed by the combination of the chromium ion, carrier protein and an antibody which can be specifically combined with the carrier protein, or a complex formed by the combination of the carrier protein and the chromium ion combined with immunoglobulin. The chromium chelating type immune complex disclosed by the invention can be used for detecting whether the chromium chelating type immune complex exists in serum of crowds at regions and the content of the chromium chelating type immune complex, and the content of the chromium chelating type immune complex indirectly reflects the chromium pollution degree of the regions.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a chromium chelated immune complex and a preparation method and application thereof. Background technique [0002] Immune complexes (IC) are the product of antigen-antibody binding. According to the definition of the National Committee for Scientific and Technical Terms, IC refers to the complex formed by the combination of antibody and soluble antigen. It is divided into two categories, one is the fixed immune complexes in the tissue, and the other is the immune complexes that are free in the blood and become circulating immune complexes (circulating immune complexes, CIC). Immune complexes play an indispensable role in clearing and destroying various antigens, and the result of the body's immune response is the continuous production of immune complexes. When the number of antigens (or antibodies) in the body is slightly more than that of antibodies (or antigens), an immune ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C07K16/00C07K1/22G01N33/53
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广州锦海骢健康科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap