Alpinia oxyphylla leaf extract and preparation method and application thereof

A kind of leaf extract and extract technology, applied in application, food preparation, food science, etc.

Active Publication Date: 2015-11-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies on the treatment of diabetes have not been reported

Method used

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  • Alpinia oxyphylla leaf extract and preparation method and application thereof
  • Alpinia oxyphylla leaf extract and preparation method and application thereof
  • Alpinia oxyphylla leaf extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The extract of Nootropia leaves was obtained by the following method: take 3400 g of dried Nomoleaf leaves, cut them into pieces, add 75V% ethanol, extract 3 times at 55°C, each time for 3 hours, and the volume of ethanol added each time is equal to the amount of completely submerged Nomoleaf. leaves as the standard, filter, combine the filtrate, concentrate and dry to obtain the total extract of Aloe nocifolia ethanol (extraction rate 23.33%); disperse the total extract in a small amount of water, and use equal volumes of petroleum ether, ethyl acetate, n-butanol extraction, the extracts were collected separately, and each extract was evaporated to dryness to obtain petroleum ether extract (extraction rate 1.41%), ethyl acetate extract (extraction rate 9.42%) and n-butanol extract ( Extraction rate 11.71%).

Embodiment 2

[0019] The extract of Pueraria chinensis is obtained by the following method: take 3400 g of dried Pueraria chinensis leaves, cut them into pieces, add 70V% ethanol, extract 3 times at 50°C, each time for 2h, and the volume of ethanol added each time is such that it can completely submerge the leaves As the standard, filter, combine the filtrates, concentrate and dry to obtain the total ethanol extract of Alpinia chinensis leaves; disperse the total extract in a small amount of water, extract with equal volumes of petroleum ether, ethyl acetate, and n-butanol in sequence, and collect the extracted extracts respectively. Each extract was evaporated to dryness to obtain petroleum ether fraction extract (extraction rate 1.23%), ethyl acetate fraction extract (extraction rate 10.11%) and n-butanol fraction extract (extraction rate 11.08%).

Embodiment 3

[0021] The extract of Nootropia leaf is obtained by the following method: Take 3400 g of dried Noelobosa leaf, cut it into pieces, add 60V% ethanol, and extract 3 times at 60°C, each time for 1h, the volume of ethanol added each time is enough to completely submerge the Nomoleaf leaf As the standard, filter, combine the filtrates, concentrate and dry to obtain the total ethanol extract of Alpinia chinensis leaves; disperse the total extract in a small amount of water, extract with equal volumes of petroleum ether, ethyl acetate, and n-butanol in sequence, and collect the extracted extracts respectively. solution, each extract was evaporated to dryness to obtain petroleum ether fraction extract (extraction rate 1.53%), ethyl acetate fraction extract (extraction rate 9.65%) and n-butanol fraction extract (extraction rate 10.96%).

[0022] Effect experiment 1: In vitro enzyme inhibitory activity test of different extracts of Yizhi leaves

[0023] The present invention selects an ...

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PUM

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Abstract

The invention belongs to the technical field of plant extraction and particularly relates to alpinia oxyphylla leaf extract and a preparation method and application thereof. The preparation method of the alpinia oxyphylla leaf extract includes: taking alpinia oxyphylla leaves, adding ethanol, heating for extraction, and concentrating and drying extracted liquid to obtain ethanol total extract; dispersing the ethanol total extract into water, sequentially using petroleum ether, ethyl acetate and n-butanol for extraction, and volatizing solvent of each extract to respectively obtain petroleum ether part extract, ethyl acetate part extract and n-butanol part extract. In vitro and in vivo experiments show that the alpinia oxyphylla leaf extract has certain hypoglycemic effect in vitro and in vivo.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an extract of leaves of edulis japonicus and its preparation method and application. Background technique [0002] Diabetes mellitus (DM) is a complex endocrine disease caused by the interaction of genetic and environmental factors, that is, relative and / or absolute insulin secretion deficiency (including β-cell decay, decreased function or / and insulin resistance), caused by sugar, A series of metabolic disorders such as protein, fat and secondary water and electrolytes. Often divided into:? type, type Ⅱ and gestational diabetes. Diabetic patients may experience impaired glucose tolerance, hyperglycemia, abnormal urine glucose and insulin release test, and the clinical manifestations are polydipsia, polyphagia, polyuria and weight loss (three more and one less), that is, polydipsia, hunger, weight loss, fatigue Symptoms such as weakness. [0003] Di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9062A61P3/10A23L1/29A61K127/00A23L33/00
Inventor 康文艺魏金凤朱晓娣何楠余琦
Owner HENAN UNIVERSITY
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