A rapid preparation method of decellularized single-lobe liver bioscaffold

Abstract

A preparation method for rapid decellularized single-lobe liver bioscaffold, without using ionic detergent SDS, which is prone to thrombus formation after tissue transplantation and low replantation rate of decellularized tissue during recellularization, and adopts weak base The decellularized left outer lobe liver bioscaffold was prepared by the perfusion method of the neutral Triton solution. The addition of NaOH in the Triton solution can make the neutral Triton solution weakly alkaline, enhance the effect of cell lysis and removal, shorten the time required for cell removal, and The optimal concentration and dosage of NaOH and Trltion solution were used to achieve the best decellularization effect.

Description

technical field [0001] The invention relates to the field of organ tissue decellularization, in particular to a method for preparing a rapid single-lobe liver decellularized biological scaffold. Background technique [0002] The extracellular matrix (ECM) is the substance that fills the cells. Its components include collagen, mucin, and proteoglycan, in addition to water, electrolytes, and a small amount of liquid phase components. They together constitute the microstructure of cell growth. environment. The local microenvironment of hepatocytes is a variety of cytokines secreted by the extracellular matrix and stromal cells, including hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), transforming growth factor (TGF-β), and platelet-derived growth factor (PDGF). Among them, ECM not only provides a three-dimensional spatial structure for the growth of intrahepatic cells, but also p...

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Problems solved by technology

[0003] Since 2010, foreign scholars have successfully prepared whole liver decellularized bioscaffolds by perfusion with different detergents[4,7-11], but there is no uniform standard for the perfusion pathway and protocol, which will affect the preparation quality and quality of decellularized liver bioscaffolds. serious impact on stability

However, as a commonly used detergent, the residue of SDS will lead to easy formation of thrombus after tissue transplantation, and the low replantation rate of decellularized tissue during recellularization[13]

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