Using ems to induce 'hort 16A' progeny superior strain variation and AFLP detection method

Abstract

The invention relates to methods for inducing 'Hort 16A' offspring superior plant variation by using EMS and AFLP detection, and relates to a kiwifruit breeding and identification method. According to the invention, the method for directly adding the EMS into a culture medium is adopted to induce kiwifruit, and the induction effect of the EMS with the concentration of 0.08 mg / L to an embryonic callus is relatively remarkable. In the AFLP, the influence of the enzyme use quantity, enzyme digestion time and enzyme activity required by PCR amplification to the enzyme digestion is researched; the influence of the enzyme activity and energy to primer connection is analyzed; appropriate primer combinations are screened out in a selective amplification system to be used as selective amplification primers after DNA pre-amplification, so that the selective amplification of polymorphic DNA is facilitated, and the reliability for reflecting the fragment length polymorphism of the polymorphic DNA is improved; therefore, the purpose of detecting the small difference of DNA levels of mutants generated through induction by one-time detection can be achieved.

Description

Technical field [0001] The invention belongs to a plant breeding and identification method, particularly a kiwi fruit breeding and identification method. Background technique [0002] Kiwifruit (kiwifruit) belongs to Actinidiacea (Actinidiacea, Actinidia spp. ), is a perennial deciduous vine, dioecious. Kiwifruit is a species of artificially domesticated and cultivated wild fruit trees. By artificially inducing mutation and mutation of kiwifruit, it can produce varieties with new flavors or new traits that are not originally available in nature or are difficult to obtain by conventional methods. However, there are few reports on EMS treatment of kiwifruit mutation breeding. Literature 1 (Long Zili, Study on in vitro regeneration and mutation induction of kiwifruit [D] . :Zhejiang University, 2008.) The callus of kiwifruit was treated with EMS to induce its adventitious shoot mutation; Literature 2 (Zhou Liming, Wang Fei, Wang Jia, EMS mutagenesis treatment directed screening of...

AI Technical Summary

Problems solved by technology

But both use EMS alone to soak the callus for a long time, and then transfer it to the medium to induce kiwifruit, which takes a long time

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