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A kind of lak cell preservation solution and method for preparing and preserving lak cell

A technology for preserving liquid and cells, applied in the field of cell culture, can solve the problems of decreased LAK cell activity, etc., and achieve the effects of safe preservation, optimal expression and simple preparation.

Active Publication Date: 2017-12-05
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, it is necessary to provide a new LAK cell preservation solution and a method for preparing and preserving LAK cells aiming at the problem of decreased cell activity of LAK cells in existing preservation solutions

Method used

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  • A kind of lak cell preservation solution and method for preparing and preserving lak cell
  • A kind of lak cell preservation solution and method for preparing and preserving lak cell
  • A kind of lak cell preservation solution and method for preparing and preserving lak cell

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Effect test

Embodiment 1

[0040] Embodiment 1, LAK cell preservation solution and preparation method thereof

[0041] Dissolve 8g of heparin sodium powder and 2.5g of Ganoderma lucidum polysaccharide freeze-dried powder with a small amount of compound electrolyte solution, then add 5mL of human serum albumin injection with a mass percentage of 20% to it, add compound electrolyte solution to 100mL and mix evenly to obtain the present invention LAK cell preservation solution. In this embodiment, the mass and volume percentages of each component are: human serum albumin 1%, heparin sodium 8%, ganoderma polysaccharide 2.5%, and the balance is compound electrolyte solution.

Embodiment 2

[0042] Embodiment 2, LAK cell preservation solution and preparation method thereof

[0043] Dissolve 5g of heparin sodium powder and 2g of Ganoderma lucidum polysaccharide freeze-dried powder with a small amount of compound electrolyte solution, then add 5mL of human serum albumin injection with a mass percentage of 20% to it, add compound electrolyte solution to 100mL and mix evenly to obtain LAK of the present invention Cell preservation solution. In this embodiment, the mass volume percentage of each component is: human serum albumin 1%, heparin sodium 5%, ganoderma polysaccharide 2%, and the balance is compound electrolyte solution.

Embodiment 3

[0044] Embodiment 3, LAK cell preservation solution and preparation method thereof

[0045] Dissolve 3g of heparin sodium powder and 5g of Ganoderma lucidum polysaccharide freeze-dried powder with a small amount of compound electrolyte solution, then add 25mL of human serum albumin injection with a mass percentage of 20%, add compound electrolyte solution to 100mL and mix evenly to obtain LAK of the present invention Cell preservation solution. In this embodiment, the mass and volume percentages of each component are: human serum albumin 5%, heparin sodium 3%, ganoderma polysaccharide 5%, and the balance is compound electrolyte solution.

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Abstract

The invention relates to an LAK cell preserving fluid and a preparation method thereof as well as a method for preparing LAK cells by adopting same. The LAK cell preserving fluid contains proserum, heparin sodium, ganoderan and preserving base solution, wherein the preserving base solution is compound electrolyte solution. LAK cells are prepared into a cell preparation by adopting any of the abovementioned LAK cell preserving fluid, so that the LAK cells can be preserved under room temperature or temperature below the room temperature. The LAK cell preserving fluid can effectively maintain the activity of the LAK cells; by adopting the LAK cell preserving fluid for preserving LAK cells for 12h under room temperature or for 72h at 4 DEG C, the activity of the cells is kept 90% or above; when the activity of the cells is maintained to be 90% or above, the killing activity of the LAK cells and the expression conditions of markers on the surfaces thereof have no obvious changes.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a LAK cell preservation solution and a method for preparing and preserving LAK cells. Background technique [0002] With the aging population, the destruction of the ecological environment, the emergence of unhealthy lifestyles and food safety issues, the incidence of cancer in my country has continued to rise for many years, and it has become a public health problem and even a social problem that must be attached great importance to. According to the World Cancer Report, in 2012, the number of cancer cases in China was 3.065 million, accounting for about one-fifth of the global incidence; the number of cancer deaths was 2.205 million, accounting for about one-quarter of the global cancer deaths. At present, the incidence and mortality of cancer in my country are showing a continuous growth trend. The incidence of cancer in my country is close to the world level, but the mortality rat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
Inventor 葛啸虎陈海佳王一飞罗二梅张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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