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antitumor peptide

An anti-tumor and tumor technology, applied in anti-tumor drugs, peptides, peptide sources, etc., can solve the problems of long peptide chain, unstable structure, easy inactivation, etc., and achieve the effect of high anti-tumor activity

Active Publication Date: 2018-03-30
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of anti-tumor peptide directly derived from the body generally has a long peptide chain, which is difficult to synthesize by chemical methods, and has the disadvantages of unstable structure and easy inactivation.

Method used

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  • antitumor peptide
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Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1 Solid phase synthesis, cleavage, purification and identification of antitumor peptides

[0018] 1.1 Solid phase synthesis of antitumor peptides

[0019] The present invention utilizes a polypeptide synthesizer to synthesize an anti-tumor peptide using a solid-phase synthesis method:

[0020] 1) With DMF as solvent, the concentration of various α-amino acids protected by Fmoc is 0.25M, the concentration of HBTU solution and HOBt solution is 0.33M, the concentration of piperidine solution is 200ml / L, and the concentration of DIEA solution is 174.2ml / L.

[0021] 2) Weigh 0.05mmol of Fmoc-Ala-Wang resin (functional group content 0.33mmol / g) into a solid-phase reactor, add 8ml of DCM to swell overnight, and remove the solvent under reduced pressure. Add 8ml of piperidine solution with a concentration of 200ml / L, react at room temperature for 5min, and drain; then add 8ml of piperidine solution with a concentration of 200ml / L, react for 20min at room temperature, ...

Embodiment 2

[0034] Example 2: Determination of Antitumor Activity of Antitumor Peptides

[0035] 1.1 MTT experiment

[0036] (1) Using human cervical cancer cells (Hela), human liver cancer cells (HepG2), human prostate cancer cells (PC-3), human liver cancer cells (HuH-7) and human salivary gland adenoids in the log phase of growth For cystic carcinoma cells (Salivary Adenoid Cysticcarcinoma, SACC-83), the cells were digested and resuspended to obtain a cell suspension, and the cells were diluted to 50,000 cells / mL. Add 100 μL to each well of the 96-well plate, put the paved 96-well plate in the incubator, and keep at 37°C, 5% CO 2 conditions for 16 hours.

[0037] (2) Dilute the polypeptide sample with medium not containing FBS. Add different concentrations of peptides to the 96-well plate. Among them, five concentration gradients of 1 μM, 2 μM, 4 μM, 8 μM, and 16 μM were set for polypeptide 1, and 6 replicate wells were set for each concentration. Peptide 5 was set at 5 concentrat...

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Abstract

The invention relates to anti-tumor peptide with the sequence of Phe-Lys-X1-Gly-Gly-Phe-X2-Lys-Lys-X3-Trp-Arg-Ser-X4-X5-Ala, wherein X1, X2, X3 and X5 are independently Leu, Ile or Val; X4 is Lys, Leu, Ile or Val.

Description

technical field [0001] The present invention relates to an anti-tumor peptide, in particular to an anti-tumor peptide with 16 amino acids. Background technique [0002] Cancer has high morbidity and mortality rates worldwide. In recent decades, human beings have made unremitting efforts to treat cancer, and the research on cancer mainly focuses on developing new treatment methods and reducing the side effects of treatment methods on patients. The commonly used cancer treatment methods are mainly surgery or chemotherapy, but in many cases not only cannot treat patients efficiently, but also have a high risk of complications. Some cancer treatment drugs not only have multiple side effects, but also may cause cause multidrug resistance. As a new type of drug for treating cancer, anti-tumor polypeptide is attracting more and more attention because it has the characteristics of being selective to tumor cells and not easy to cause drug resistance. [0003] Many antitumor peptid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61K38/17A61P35/00A61P35/02
CPCA61K38/00C07K14/43522
Inventor 罗施中高红柳田希博周希蕊张强
Owner BEIJING UNIV OF CHEM TECH
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