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Maize zmERF18 gene promoter and application thereof

A promoter and corn technology, applied in the field of genetic engineering, to achieve the effect of improving water resistance, important theoretical value and practical significance

Inactive Publication Date: 2015-11-18
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cloning of the maize ERF gene promoter has not been reported yet.

Method used

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  • Maize zmERF18 gene promoter and application thereof
  • Maize zmERF18 gene promoter and application thereof

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Embodiment Construction

[0012] The first aspect of the present invention provides a maize zmERF18 gene promoter, the nucleotide sequence of which is shown in SEQ ID No: 1, the full length of the sequence is 1390bp, and the promoter contains 1 GARE, 1 TC-richmotif, 3 Skn-1motif, 1 CGTCA-motif and 1 MBS site cis-element, these cis-elements are related to plant hormones, anaerobic stress response; is a tissue-specific promoter, only expressed in roots; is an inducible promoter submerged, high expression induced by flooding.

[0013] The maize zmERF18 gene promoter also includes a DNA fragment with the same function derived from the nucleotide sequence shown in SEQ ID No: 1, which is substituted, deleted or added by one or several nucleotides.

[0014] The maize zmERF18 gene promoter was cloned from the maize inbred line Hz32.

[0015] The second aspect of the present invention provides a vector containing the promoter of the maize zmERF18 gene. In the embodiment of the present invention, the vector co...

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Abstract

The invention provides a maize zmERF18 gene promoter. The maize zmERF18 gene promoter is characterized by comprising nucleotide sequences shown as SEQ ID No.1 or certain nucleotide sequences. The certain nucleotide sequences have functions equivalent to functions of the nucleotide sequences shown as the SEQ ID No.1 and are derived from the nucleotide sequences shown as the SEQ ID No.1 by means of replacing, omitting or adding a nucleotide or a plurality of nucleotides. The maize zmERF18 gene promoter has the advantages that transgenic plants with exogenous genes can be directly driven by the promoter, the promoter is high in promotion ability, the exogenous genes can be quickly and effectively promoted to be strongly expressed at the roots of the plants under flooding conditions, and the waterlogging tolerance or the insect resistance, the disease resistance and the like of the plants can be improved; the promoter is a tissue specific promoter which is only specifically expressed at roots, and accordingly the transgenic food safety problem of the transgenic plants with edible non-root portions can be solved; novel root specific expression and flooding inducible promoter resources can be provided for modifying waterlogging tolerance inheritance of maize and other arid crop, molecular mechanisms for forming the waterlogging tolerance of the maize can be further illustrated, and accordingly the maize zmERF18 gene promoter has important theoretical value and an important practical significance.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a maize zmERF18 gene promoter and its application. Background technique [0002] By the middle of this century, the global population will reach 9 billion, and there is an urgent need to increase and stabilize crop yields to meet the growing needs of the population. Corn is the crop with the largest planting area in my country. However, in southern China and Southeast Asia, flood disasters in some areas have brought serious losses to corn production. In South Asia alone, more than 15% of the maize acreage suffers varying degrees of damage each year. In India, floods have reduced maize yields by 25-30% annually. In the normal cultivation season, spring maize in southern China and summer maize in northern my country often suffer from floods and waterlogging, and the root system of maize is in a state of low oxygen for a long time, resulting in a decrease of 10%-40% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00
Inventor 杜何为黄敏
Owner YANGTZE UNIVERSITY
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