Breeding method for hybrid rice
A hybrid rice and rice technology, applied in the field of hybrid rice breeding, can solve the problems of reducing the income of seed producers, increasing the cost of seed production, affecting the quality of commercial grains, etc. The effect of improving the efficiency of seed production
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Embodiment 1
[0042] The breeding of embodiment 1 hybrid rice
[0043] 1. Select large-grain (GW=0.6cm) (NRIL16, selected by the Rice Research Institute of Anhui Academy of Agricultural Sciences using the F4 generation of Chuandali / Yangdao 6, bred and sold by the Rice Research Institute of Anhui Academy of Agricultural Sciences) and small-grain rice ( Chuan 7, preserved by the Rice Research Institute of Anhui Academy of Agricultural Sciences, a local variety in Sichuan, GL=0.5cm) germplasm;
[0044] 2. Using the excellent two-line GMS line N876S (Guangzhan 63S) as the female parent, crossed with Minghui 63, backcrossed and self-crossed (BC 1 f 3 ), the obtained two-line sterile line, self-bred by the Rice Research Institute of Anhui Academy of Agricultural Sciences and sold to the outside world) as the female parent and hybridized with rice argentina, and N876S was used as the recurrent parent to backcross for 2-3 generations and then self-crossed.
[0045] Hefei, planting BC in early May...
Embodiment 2
[0054] The breeding of embodiment 2 hybrid rice
[0055] 1. Use 0.5% EMS to mutate the excellent two-line GMS line N876S, plant the mutagenized M1 generation (about 30,000 plants), and harvest the seeds of the mutagenized M1 plant (M2 generation, no less than 50 seeds per plant).
[0056] 2. In Hefei, in early May, plant the M2 generation seeds into rows (no less than 30 rows per row), select small-grained, and microscopically inspected individual plants with excellent agronomic properties, transplant and regenerate individual plants without pollen after stubble cutting, and harvest Seeds on regenerated rice.
[0057] 3. In the same year, Hainan, planted in mid-November, selected individual plants with small grains and excellent agronomic properties for selfing, and harvested the seeds.
[0058] 4. In the next year, Hefei, plant the seeds harvested in step 3 in early May, select a single plant with small grains and excellent agronomic properties, microscopically check, transp...
Embodiment 1
[0066] Breeding of comparative example 1 hybrid rice
[0067] 1. Select the germplasm of large-grain rice (thousand-grain weight above 40-60g) and small-grain rice (thousand-grain weight above 15g), which are Sichuan Dali (germplasm resources provided by Sichuan Rice and Sorghum Research Institute), Xiaoxiangai (Hunan Rice Research Institute) provided rice seed resources);
[0068] 2. In Changsha, the two-line GMS line Y58S was used as the female parent to cross with the large-grain rice Chuan Da-grain to obtain F 1 planted as F 2 Genetic population, select a number of excellent sterile individual plants with grain shape similar to Chuan Da grain from the population, and obtain F 3 seed.
[0069] In the same year, F 3 For generation, select excellent individual plants with a thousand-grain weight above 35g from the population, and harvest F 4 seed.
[0070] 3. Bring back to Changsha to plant F next year 4 Group, select excellent individual plants with a thousand-grain w...
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