Noninvasive prenatal screening 2, 1-trisomy syndrome kit
A technique for trisomy syndrome and prenatal screening, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc. It can solve the problems that the sensitivity needs to be further improved, it is easy to cause intrauterine infection, and the operation is traumatic. Achieve the effects of preventing the birth of new children, shortening the detection cycle, and high sensitivity
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Embodiment 1
[0023] A non-invasive prenatal screening kit for trisomy 21, comprising a PCR reaction solution, the PCR reaction solution contains probes and primers, the probes are TaqMamUPL6-FAM probes and TaqMamUPL15-TET probes, TaqMamUPL6-FAM The probe is used to detect chromosome 18, and the TaqMamUPL15-TET probe is used to detect chromosome 21. For chromosome 21, 20 pairs of corresponding upstream primers and downstream primers are designed and synthesized. The gene sequence is SEQ ID NO.1-40; and Design and synthesize 20 pairs of corresponding upstream primers and downstream primers for chromosome 18, whose gene sequence is SEQ ID NO.41-80; TaqMamUPL6-FAM probe, whose gene sequence is SEQ ID NO.81; TaqMamUPL15-TET probe, whose gene sequence The sequence is SEQ ID NO.82.
[0024] The PCR reaction solution also contains extracted maternal peripheral blood fetal cfDNA, TaqManGenotypingMasterMix, droplet stabilizer and ultrapure water.
[0025] The final concentration of TaqManGenotyping...
Embodiment 2
[0026] Example 2 The application of the non-invasive prenatal screening kit for trisomy 21, comprising the following steps:
[0027] Step 1: collect peripheral EDTA anticoagulant blood of pregnant women, and extract free DNA from peripheral blood of pregnant women;
[0028] 2: Use primer design software to independently design primers to prepare water-in-oil droplets;
[0029] 3: 20-fold digital PCR single-molecule amplification;
[0030] 4: Optical signal detection and counting of PCR products;
[0031] 5: Perform bioinformatics analysis on the results to obtain fetal genetic data.
[0032] Specifically include the following steps:
[0033] 10mL of peripheral blood was collected from the hospital for 5 high-risk pregnant women who were tested for Tang screening (these 5 pregnant women were diagnosed with trisomy 21 by drawing amniotic fluid in the hospital at the same time, and two of them were diagnosed as trisomy 21 , three normal fetuses), follow the steps below for no...
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