A non-invasive prenatal screening kit for trisomy 21-

Abstract

The invention discloses a noninvasive prenatal screening 2, 1-trisomy syndrome kit, and relates to the technical field of a nucleic acid determination or inspection method. The noninvasive prenatal screening 2, 1-trisomy syndrome kit comprises a PCR reaction solution, wherein the PCR reaction solution contains a TaqMam UPL6-FAM probe and a TaqMam UPL15-TET probe, the TaqMam UPL6-FAM probe and the TaqMam UPL15-TET probe are respectively used for detecting No.18 and No.21 chromosomes, 20 pairs of primers are respectively designed for the No.21 and No.18 chromosomes, gene sequences of the primers are SEQ ID NO.1 to 40 and SEQ ID NO.41-80; and gene sequences of the TaqMam UPL6-FAM probe and the TaqMam UPL15-TET probe are SEQ ID NO.81 and SEQ ID NO.82. By adopting the noninvasive prenatal screening 2, 1-trisomy syndrome kit, the noninvasive prenatal screening of 21-trisomy syndrome, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of nucleic acid determination or detection methods, in particular to a non-invasive prenatal screening kit for trisomy 21. Background technique [0002] Trisomy 21 is the most common chromosomal disease in the fetus. The incidence rate in newborns is about 1 / 800, accounting for 70-80% of chromosomal diseases in children. The incidence rate increases with the increase of mother's age. At present, there are more than 1 million patients in my country, and a child with trisomy 21 syndrome is born in an average of 20 minutes. There are as many as 270,000 children with trisomy 21 syndrome born in the country every year. Patients with trisomy 21 have an extra chromosome 21, which mainly manifests as mental retardation, developmental delay and special facial features. Because trisomy 21 has brought irreparable pain and heavy mental burden to parents, families and society, prenatal diagnosis for high-risk groups suc...

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned prenatal genetic diagnosis includes cytogenetic diagnosis based on invasive procedures such as amniocentesis and chorionic villus aspiration. Although the diagnosis is accurate, it is easy to cause intrauterine infection, miscarriage, and even harm to the fetus because of the traumatic operation. Has a certain impact and is an intrusive inspection

Although high-throughput sequencing methods and other non-invasive methods have solved the problems caused by invasiveness, the operation is relatively cumbersome and the sensitivity needs to be further improved.

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