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Molecular Breeding Method of Chestnut Varieties with Short Stamens

A short male inflorescence and molecular breeding technology, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of long selection period and low selection accuracy, and improve hybridization efficiency , the effect of reducing the amount of hybrid labor

Active Publication Date: 2017-07-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

[0003] In fruit tree breeding, traditional breeding is mainly based on the evaluation and selection of plants in the field. However, due to the influence of the environment, the selection accuracy is not high, and most of the traits need to be observed after many years, so the selection cycle is long.
With the rapid development of genomics, plant molecular breeding has been applied in a variety of crops. At present, there are few reports on the practice of chestnut molecular breeding

Method used

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  • Molecular Breeding Method of Chestnut Varieties with Short Stamens
  • Molecular Breeding Method of Chestnut Varieties with Short Stamens
  • Molecular Breeding Method of Chestnut Varieties with Short Stamens

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Development of Molecular Markers Related to Chestnut Short Stamina Characters

[0038]Using the SLAF-seq (Specific-Locus Amplified Fragment Sequencing) technology developed by Beijing Biomike Biotechnology Co., Ltd. to carry out association analysis on the genetic segregation population of chestnut short male flowers, the molecular markers closely related to chestnut short male flowers were obtained. The specific process is as follows: use the chestnut genome to identify SNPs through bwa comparison software and GATK software. And filter the SNP. The filtering method is as follows: first filter the sites with a read support degree of less than 3, and then filter the sites in the offspring mixed pool that differ from the SNP types of the corresponding parents, and finally obtain high-quality credible SNP sites, and identify them on this basis The point of difference between the two pools. Finally, association analysis was carried out using the loci of differen...

Embodiment 4

[0064] Example 4 The method of cultivating new varieties of Chinese chestnut with insect resistance and storage resistance and short male inflorescence

[0065] The method comprises the steps of:

[0066] 1) Configure the hybrid combination: take the chestnut variety 'Heishanzhai No. 7' as the female parent, and select excellent germplasm resources such as 'Extra Early Maturity', 'Dayouli', 'Gushi No. 4' as the male parent, collect pollen and mix 'Heishanzhai 7' was pollinated. In the pollination process, 'Heishanzhai 7' did not need to be detasseled and bagged. The true hybrids were screened with primer I, and the F1 generation was obtained by hybridization.

[0067] 2) Constructing the gene haplotype molecular ID card of the hybrid parent: same as step 2) of Example 2.

[0068] 3) Molecular marker screening of hybrid seedlings: using the 'Gushi No. 4' haplotype molecular ID card and the molecular markers developed in Example 1 related to the chestnut short male inflorescenc...

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Abstract

The present invention provides a method for molecular breeding of chestnut varieties with short stamens, comprising: 1) using 'Heishanzhai No. 7' as the female parent and chestnut germplasm resources with excellent traits as the male parent, collecting pollen for 'Heishanzhai No. 7' 'Pollination; 2) Analyze the sequence differences of chestnut genes among the hybrid parents through sequencing technology, and construct the gene haplotype molecular ID cards of each hybrid parent based on the differential sequence; 3) Develop molecular markers related to the short male inflorescence traits of chestnut; 4) Using the genetic haplotype molecular ID card constructed above and the molecular markers related to the trait of short male inflorescence of chestnut, respectively screen the F1 generation hybrid seedlings, so as to obtain the hybrid seedlings with both short male inflorescence gene and excellent germplasm background. hybrid seedlings. The present invention realizes the purpose of rapidly aggregating chestnut short stamen genes and other excellent genes in the F1 generation of hybrids through the combination of molecular marker-assisted selection and conventional breeding means.

Description

technical field [0001] The invention relates to the field of molecular breeding, in particular to a method for molecular breeding of short stamen chestnut varieties. Background technique [0002] Chestnut occupies a very important position in the forest and fruit production in mountainous areas of Beijing. According to statistics in 2010, chestnut is the tree species with the largest cultivated area, exceeding 600,000 mu, with an output value of 220 million yuan, accounting for 40% of the total output value of dried fruits. Chestnut male inflorescence is a catkin, and the normal male inflorescence is about 17cm long, and the amount of flowers is very large. It consumes 20-40% of the water and nutrition of the tree body, which is one of the important reasons for the low yield of chestnut trees. Therefore, cultivating chestnut varieties with short stamens is of great significance in chestnut production. [0003] In fruit tree breeding, traditional breeding is mainly based on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02C12Q1/68
Inventor 胡广隆黄武刚程丽莉张杰伟兰彦平曹庆昌
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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