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Molecular breeding method of short catkin chestnut varieties

A short male inflorescence, molecular breeding technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, can solve the problems of long selection period and low selection accuracy, and improve hybridization efficiency. , the effect of reducing the amount of hybrid labor

Active Publication Date: 2015-12-16
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

[0003] In fruit tree breeding, traditional breeding is mainly based on the evaluation and selection of plants in the field. However, due to the influence of the environment, the selection accuracy is not high, and most of the traits need to be observed after many years, so the selection cycle is long.
With the rapid development of genomics, plant molecular breeding has been applied in a variety of crops. At present, there are few reports on the practice of chestnut molecular breeding

Method used

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  • Molecular breeding method of short catkin chestnut varieties
  • Molecular breeding method of short catkin chestnut varieties
  • Molecular breeding method of short catkin chestnut varieties

Examples

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Embodiment 1

[0037] Example 1 Development of Molecular Markers Related to Chestnut Short Stamina Characters

[0038]The SLAF-seq (Specific-Locus Amplified Fragment Sequencing) technology developed by Beijing Biomark Biotechnology Co., Ltd. was used to conduct association analysis on the genetic segregation population of chestnut short male flowers, and obtain molecular markers closely related to chestnut short male flowers. The specific process is as follows: use the chestnut genome to identify SNPs through bwa comparison software and GATK software. And filter the SNP. The filtering method is as follows: first filter the sites with a read support degree of less than 3, and then filter the sites in the offspring mixed pool that differ from the SNP types of the corresponding parents, and finally obtain high-quality credible SNP sites, and identify them on this basis The point of difference between the two pools. Finally, association analysis was carried out using the loci of differences be...

Embodiment 4

[0064] Example 4 The method of cultivating new varieties of Chinese chestnut with insect resistance and storage resistance and short male inflorescence

[0065] The method comprises the steps of:

[0066] 1) Configure the hybrid combination: take the chestnut variety 'Heishanzhai No. 7' as the female parent, and select excellent germplasm resources such as 'Extra Early Maturity', 'Dayouli', 'Gushi No. 4' as the male parent, collect pollen and mix 'Heishanzhai 7' was pollinated. In the pollination process, 'Heishanzhai 7' did not need to be detasseled and bagged. The true hybrids were screened with primer I, and the F1 generation was obtained by hybridization.

[0067] 2) Constructing the gene haplotype molecular ID card of the hybrid parent: same as step 2) of Example 2.

[0068] 3) Molecular marker screening of hybrid seedlings: using the 'Gushi No. 4' haplotype molecular ID card and the molecular markers developed in Example 1 related to the chestnut short male inflorescenc...

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Abstract

The invention provides a molecular breeding method of short catkin chestnut varieties. The method comprises the following steps that (1) Heishanzhai No.7 is used as a female parent, chestnut germplasm resources with excellent characters are used as a male parent, and pollen is collected for performing pollination on the Heishanzhai No.7; (2) the sequence differences of chestnut gene among cross parents are analyzed by a sequencing technique, and a gene haploid type molecular identity card of each cross parent is built on the basis of difference sequences; (3) molecular markers relevant to the short catkin characters are developed; (4) F1 generation of crossbred seedlings are screened by utilizing the built gene haploid type molecular identity card and the molecular markers relevant to the short catkin characters, so that crossbred seedlings with short catkin genes and excellent germplasm resource backgrounds are obtained. The molecular breeding method has the advantage that the goal of fast polymerizing the short catkin genes and other excellent genes in crossbred F1 generation is realized in a mode of combining molecular marker auxiliary selection and conventional breeding measures.

Description

technical field [0001] The invention relates to the field of molecular breeding, in particular to a method for molecular breeding of short stamen chestnut varieties. Background technique [0002] Chestnut occupies a very important position in the forest and fruit production in mountainous areas of Beijing. According to statistics in 2010, chestnut is the tree species with the largest cultivated area, exceeding 600,000 mu, with an output value of 220 million yuan, accounting for 40% of the total output value of dried fruits. Chestnut male inflorescence is a catkin, and the normal male inflorescence is about 17cm long, and the amount of flowers is very large. It consumes 20-40% of the water and nutrition of the tree body, which is one of the important reasons for the low yield of chestnut trees. Therefore, cultivating chestnut varieties with short stamens is of great significance in chestnut production. [0003] In fruit tree breeding, traditional breeding is mainly based on ...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68
Inventor 胡广隆黄武刚程丽莉张杰伟兰彦平曹庆昌
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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