Halotolerant denitrification compound inoculant and preparation method and application thereof

A compound bacterial agent and denitrification technology, which can be used in biochemical equipment and methods, chemical instruments and methods, and microorganism-based methods, etc., can solve problems such as pollution, and achieve the effects of strong adaptability, good removal effect and cost saving.

Active Publication Date: 2015-12-16
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a high-efficiency salt-tolerant and denitrification compound bacterial agent and its preparation method and application, in order to effectively solve the pollution problem caused by high nitrogen in rural domestic sewage

Method used

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  • Halotolerant denitrification compound inoculant and preparation method and application thereof
  • Halotolerant denitrification compound inoculant and preparation method and application thereof
  • Halotolerant denitrification compound inoculant and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 composite bacterial agent

[0033]1) Solid slant culture: transfer C2 and C30 to solid medium respectively, and culture them in a sterile environment at 30°C for 48 hours to activate the strains. Wherein, the solid medium of C2 is: ammonium sulfate 0.5g / L, succinic acid (sodium succinate) 2.17g / L, trace element solution 50mL, pH7.0~7.2; The composition of described trace element solution is: K 2 HPO 4 .3H 2 O6.5g / L, MgSO 4 .7H 2 O2.5g / L, NaCl2.5g / L, FeSO 4 .7H 2 O0.05g / L, MnSO 4 .H 2 O0.04g / L, agar 20g / L; C30 solid medium: yeast extract powder 5g / L, tryptone 10g / L, NaCl50g / L, pH7.0~7.5, agar 20g / L.

[0034] 2) Seed culture: inoculate the activated strains in step 1) into liquid medium respectively, and culture with shaking at 30° C. and 160 rpm for 48 hours until the strains are in the logarithmic growth phase. Wherein, the liquid culture medium of C2 is: ammonium sulfate 0.5g / L, succinic acid (sodium succinate) 2.17g / L, trace el...

Embodiment 2

[0037] Example 2 Application of composite bacterial agent in wastewater (sewage) of a farmhouse in Yuqiao Reservoir, Tianjin

[0038] (1) Pre-treatment of wastewater: Take wastewater from a farmhouse in Yuqiao Reservoir, Tianjin, and pass through a 30-mesh grid to remove suspended solids with larger diameters in the water. After testing: the wastewater pH is 7.2, the ammonia nitrogen concentration is 70.6mg / L, and the CODcr is 320mg / L. At the same time, NaCl was added to the waste water to maintain the salt content in the waste water at 3%.

[0039] (2) Take 5L of treated waste water and inoculate the bacterial agent in two ways.

[0040] Method 1: inoculate the single strains of C2 and C30 into the wastewater respectively, and the inoculum amount is 10% by volume.

[0041] Mode 2: The composite microbial agent obtained in Example 1 is inserted into the waste water according to the inoculation amount of 10% volume.

[0042] Two inoculation methods, the culture temperature i...

Embodiment 3

[0044] Example 3 Application of composite bacterial agent in waste water (sewage) of a breeding factory in Yangliuqing, Tianjin

[0045] (1) Pre-treatment of wastewater: Take wastewater from a breeding factory in Yangliuqing, Tianjin, and pass it through a 30-mesh grid to remove suspended solids with larger diameters in the water. After testing: the wastewater pH is 8.2, the ammonia nitrogen concentration is 443mg / L, and the COD is 3260mg / L. At the same time, NaCl was added to the waste water to maintain the salt content in the waste water at 3%.

[0046] (2) Take 5L of treated waste water and inoculate the bacterial agent in two ways.

[0047] Method 1: inoculate the single strains of C2 and C30 into the wastewater respectively, and the inoculum amount is 10% by volume.

[0048] Mode 2: The composite microbial agent obtained in Example 1 is inserted into the waste water according to the inoculation amount of 10% volume.

[0049] Two inoculation methods, the culture tempera...

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Abstract

The invention relates to the field of rural domestic sewage treatment, in particular to a halotolerant denitrification compound inoculant and a preparation method and application thereof. The halotolerant denitrification compound inoculant comprises Hydrogenophaga pseudoflava sp.C2 and Virgibacillus halodenitrificans sp.C30 which are collected at the China General Microbiological Culture Collection Center (CGMCC for short) on 10th June, 2015 and under serial numbers of CGMCC No.10977 and CGMCC No.10978. The two microorganisms contained in the compound inoculant are screened from native microorganisms in rural domestic sewage, and the compound inoculant prepared is high in adaptability and has a function of one-time bacterium throwing for long-time use, so that cost is saved greatly; safety of a bacterium strain does not need to be considered, the compound inoculant is suitable for rural areas where gathering places are dispersive in China.

Description

technical field [0001] The invention relates to the field of rural domestic sewage treatment, in particular to a microbial composite microbial agent for biologically treating rural domestic sewage and having the functions of denitrification and salt tolerance. Background technique [0002] China currently has more than 600,000 administrative villages with a population of 760 million, 96% of which lack sewage treatment systems. Rural sewage has a large base, but affected by the level of rural economic development, production, and lifestyle, the characteristics of rural sewage discharge in different regions vary greatly. The current technology and operation mode adopted by urban sewage treatment plants are not suitable for the treatment of rural decentralized sewage. As the country intensifies the development of rural areas, such as farmhouses, village-run enterprises and breeding enterprises, the types of rural sewage continue to increase, and the content of salt, nitrogen a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 陈昢圳任天志郑向群成卫民
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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