Method for identifying phytophthora parasitica var nicotianae physiological strains

Abstract

The invention discloses a method for identifying phytophthora parasitica var nicotianae physiological strains, and belongs to the technical field of plant pathogen identification. According to the method, a tissue culture vessel method is used for identifying the phytophthora parasitica var nicotianae physiological strains; the environment conditions of culture and impregnation are more stable; the growth of tobacco pathogens is favorably accelerated; the seedling culture period and the dip dyeing period are greatly shortened; meanwhile, the interference of infectious microbes and other physiological strains on the identification result can also be avoided; the flux is high; labor and materials can be saved to the greatest degree; the high-flux, fast and accurate identification on the phytophthora parasitica var nicotianae physiological strains is realized. Compared with a land method and a greenhouse method, the method has the advantages that the identification is fast; the result is more accurate, stable and reliable; higher application values are realized; meanwhile, a thought can also be provided for the identification of physiological strains of other disease pathogenic bacteria of tobaccos.

Description

technical field [0001] The invention relates to a method for identifying physiological races of tobacco blackleg pathogen, belonging to the technical field of plant pathogen identification. Background technique [0002] Tobacco black shank is a devastating soil-borne oomycete disease worldwide. In the late 1980s, with the continuous expansion of tobacco planting area in my country, tobacco black shank disease spread rapidly. Due to the differences in the pathogenicity of different races of Tobacco black shank, close monitoring of the races of Black shank in tobacco fields is required. So far, there are 5 physiological races of Tobacco black shank pathogens reported in the world, of which 4 are reported in the United States, namely race 0, 1, 3 and 4, and 1 species is reported in Africa, namely race 2 No. 1 race, but only No. 0 and No. 1 races are found in my country. [0003] The invention patent with publication number CN101671720A discloses a method for identifying physi...

AI Technical Summary

Problems solved by technology

Although this method can preliminarily identify the physiological race of Tobacco black shank pathogen, the accuracy of the test results still needs to be verified because the field cultivation is greatly affected by environmental factors.

Generally, the identification of physiological races of tobacco black shank is carried out by root inoculation under greenhouse conditions, but this method needs to be equipped with greenhouse facilities, and the strain inoculation requires at least 7 to 8 week-old tobacco seedlings (seedlings need to be transplanted before inoculation) , time-consuming operation

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