Method for identifying phytophthora parasitica var nicotianae physiological strains

A technology of tobacco blackleg bacteria and physiological races, which is applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of time-consuming and laborious operation, and the accuracy of test results needs to be verified, and achieves high application value and shortens the time. Immersion cycle, high throughput effect

Pending Publication Date: 2015-12-23
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

Although this method can preliminarily identify the physiological race of Tobacco black shank pathogen, the accuracy of the test results still needs to be verified because the field cultivation is greatly affected by environmental factors.
Generally, the identification of physiological races of tobacco

Method used

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  • Method for identifying phytophthora parasitica var nicotianae physiological strains
  • Method for identifying phytophthora parasitica var nicotianae physiological strains
  • Method for identifying phytophthora parasitica var nicotianae physiological strains

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Embodiment 1

[0029] The identification method of tobacco black shank pathogen physiological race in the present embodiment may further comprise the steps:

[0030] (1) Response of different differential hosts to various physiological races of Tobacco blackleg

[0031] a. Soak the seeds of differentiating hosts (i.e. tobacco varieties L8, NC1071, Florida301 and Xiaojin 1025) in sterile water for 100 h, dry the surface moisture and dry naturally; soak the seeds back to dry with 70% ethanol Disinfect for 30 seconds, then disinfect with 10% sodium hypochlorite for 15 minutes, and finally wash with sterile water 4 times to remove the residual sodium hypochlorite on the surface of the seeds;

[0032] b. Under sterile conditions, the sterilized differentiated host seeds were inoculated into tissue culture bottles filled with MS medium respectively, and each tissue culture bottle was inoculated with one seed (4 replicates were established for each tobacco variety, each replicate 10 tissue culture...

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Abstract

The invention discloses a method for identifying phytophthora parasitica var nicotianae physiological strains, and belongs to the technical field of plant pathogen identification. According to the method, a tissue culture vessel method is used for identifying the phytophthora parasitica var nicotianae physiological strains; the environment conditions of culture and impregnation are more stable; the growth of tobacco pathogens is favorably accelerated; the seedling culture period and the dip dyeing period are greatly shortened; meanwhile, the interference of infectious microbes and other physiological strains on the identification result can also be avoided; the flux is high; labor and materials can be saved to the greatest degree; the high-flux, fast and accurate identification on the phytophthora parasitica var nicotianae physiological strains is realized. Compared with a land method and a greenhouse method, the method has the advantages that the identification is fast; the result is more accurate, stable and reliable; higher application values are realized; meanwhile, a thought can also be provided for the identification of physiological strains of other disease pathogenic bacteria of tobaccos.

Description

technical field [0001] The invention relates to a method for identifying physiological races of tobacco blackleg pathogen, belonging to the technical field of plant pathogen identification. Background technique [0002] Tobacco black shank is a devastating soil-borne oomycete disease worldwide. In the late 1980s, with the continuous expansion of tobacco planting area in my country, tobacco black shank disease spread rapidly. Due to the differences in the pathogenicity of different races of Tobacco black shank, close monitoring of the races of Black shank in tobacco fields is required. So far, there are 5 physiological races of Tobacco black shank pathogens reported in the world, of which 4 are reported in the United States, namely race 0, 1, 3 and 4, and 1 species is reported in Africa, namely race 2 No. 1 race, but only No. 0 and No. 1 races are found in my country. [0003] The invention patent with publication number CN101671720A discloses a method for identifying physi...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/04
Inventor 胡利伟刘芳奚家勤尹启生宋纪真薛超群王广山刘阳牟文君郭建华
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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