Mycobacterium tuberculosis antigens, nucleotide sequences and application thereof

Abstract

The invention relates to 12 mycobacterium tuberculosis antigen polypeptides, and antigen epitope polypeptides having polypeptide sequences having at least 90% of the same as each other; the antigen polypeptides or the antigen epitope polypeptides are matched with an immunologic adjuvant to obtain mycobacterium tuberculosis vaccines; the antigen polypeptides or the antigen epitope polypeptides are used as antigens, and monoclonal antibodies are prepared and used for detecting whether the sample contains mycobacterium tuberculosis; nucleic acid sequences of the 12 mycobacterium tuberculosis antigen polypeptides and polynucleotides having the nucleic acid sequences having at least 90% of the same as each other are encoded; gene vaccines containing the nucleic acids and the polynucleotides are prepared. Beneficial effects comprise that the polypeptides can be used for recombined vaccine polypeptides, or be used for development of antigen diagnostic reagents, so as to be beneficial for detection and diagnosis of pulmonary tuberculosis.

Description

technical field [0001] The invention relates to a Mycobacterium tuberculosis antigen, nucleic acid sequence and application thereof. Background technique [0002] Mycobacterium tuberculosis, the pathogen of tuberculosis, is a chronic infectious disease that kills about 3 million people every year. An estimated two billion people are infected with M. tuberculosis worldwide, including 7.5 million active TB cases. In recent years there has been an unexpected rise in tuberculosis cases. [0003] In China, there are 330 million people infected with tuberculosis and 6 million tuberculosis patients. Every year, 250,000 patients die from tuberculosis, which is twice the sum of deaths from various infectious diseases. Therefore, my country has upgraded tuberculosis from a Class C infectious disease to a Class B infectious disease and included it in the scope of strict management. How to diagnose and treat quickly and early has become a key link in controlling the spread and spread...

AI Technical Summary

Problems solved by technology

[0008] Traditional vaccine technology often produces varying degrees of immunogenicity in different hosts

Images