Mycobacterium tuberculosis antigens, nucleotide sequences and application thereof

A technology of Mycobacterium tuberculosis and nucleotide sequence, which is applied in the field of Mycobacterium tuberculosis antigen to achieve the effect of facilitating detection and diagnosis

Inactive Publication Date: 2016-01-06
邓林红 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Traditional vaccine technology often produ...

Method used

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  • Mycobacterium tuberculosis antigens, nucleotide sequences and application thereof
  • Mycobacterium tuberculosis antigens, nucleotide sequences and application thereof
  • Mycobacterium tuberculosis antigens, nucleotide sequences and application thereof

Examples

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Embodiment Construction

[0017] The present invention will now be further described in conjunction with specific examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention.

[0018] 1. Isolation of Mycobacterium tuberculosis diagnostic specific antigen

[0019] (1) Search the Mycobacterium tuberculosis polypeptide library to determine 3200 antigenic targets for humoral immunity in humans.

[0020] (2) expressing the whole polypeptide library of Mycobacterium tuberculosis by using a cell-free protein expression system;

[0021] (3) The expressed Mycobacterium tuberculosis polypeptide was separated by magnetic bead method, and the above protein was subjected to polyacrylamide electrophoresis and western blot transfer; The serum was incubated for 1 h; after that, an alkaline phosphatase-conjugated secondary antibody was added to screen for antigens that could react with serum from tuberculosis patients. The results showed that o...

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Abstract

The invention relates to 12 mycobacterium tuberculosis antigen polypeptides, and antigen epitope polypeptides having polypeptide sequences having at least 90% of the same as each other; the antigen polypeptides or the antigen epitope polypeptides are matched with an immunologic adjuvant to obtain mycobacterium tuberculosis vaccines; the antigen polypeptides or the antigen epitope polypeptides are used as antigens, and monoclonal antibodies are prepared and used for detecting whether the sample contains mycobacterium tuberculosis; nucleic acid sequences of the 12 mycobacterium tuberculosis antigen polypeptides and polynucleotides having the nucleic acid sequences having at least 90% of the same as each other are encoded; gene vaccines containing the nucleic acids and the polynucleotides are prepared. Beneficial effects comprise that the polypeptides can be used for recombined vaccine polypeptides, or be used for development of antigen diagnostic reagents, so as to be beneficial for detection and diagnosis of pulmonary tuberculosis.

Description

technical field [0001] The invention relates to a Mycobacterium tuberculosis antigen, nucleic acid sequence and application thereof. Background technique [0002] Mycobacterium tuberculosis, the pathogen of tuberculosis, is a chronic infectious disease that kills about 3 million people every year. An estimated two billion people are infected with M. tuberculosis worldwide, including 7.5 million active TB cases. In recent years there has been an unexpected rise in tuberculosis cases. [0003] In China, there are 330 million people infected with tuberculosis and 6 million tuberculosis patients. Every year, 250,000 patients die from tuberculosis, which is twice the sum of deaths from various infectious diseases. Therefore, my country has upgraded tuberculosis from a Class C infectious disease to a Class B infectious disease and included it in the scope of strict management. How to diagnose and treat quickly and early has become a key link in controlling the spread and spread...

Claims

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Application Information

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IPC IPC(8): C07K14/35C12N15/31A61K39/04A61K48/00A61P31/06G01N33/569
CPCC07K14/35A61K39/04A61K2039/53A61K2039/55561A61K2039/57G01N33/5695G01N2333/35
Inventor 徐东昕邓林红
Owner 邓林红
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