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A kind of propagation method of purple leaf thick plum

The technology of a purple leaf thick plum and the propagation medium is applied in the field of purple leaf thick plum propagation, which can solve the problems such as poor effect of purple leaf thick plum and failure of large-scale propagation of purple leaf thick plum, so as to improve the greening effect and increase the application value , the effect of high germination rate

Inactive Publication Date: 2017-09-12
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But at present, the effect of the method of breeding and cultivating Prunus japonica is poor, and it cannot effectively carry out large-scale propagation of Prunus japonica

Method used

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  • A kind of propagation method of purple leaf thick plum
  • A kind of propagation method of purple leaf thick plum
  • A kind of propagation method of purple leaf thick plum

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] Specific embodiment one: a kind of purple leaf thick plum propagation method of the present embodiment is carried out according to the following steps:

[0022] 1. Explant selection:

[0023] Select the axillary buds at the end of April as explants; first wash them with clean water, soak them in 75% ethanol by volume for 30 seconds, take out the clean water and rinse them 3 to 5 times, and then soak them in 0.5% sodium hypochlorite by volume for 10 minutes , and then rinsed with sterile water 3 to 5 times;

[0024] 2. Induction of explant buds:

[0025] Cut the explants treated in step 1 into 1-2cm stem segments and place them in the explant bud induction medium at a temperature of 20-22°C, light intensity of 2300lx, light of 12-15h / d, and culture for 30d ; Wherein, the explant bud induction medium is MS+6-BA 1.5mg / L+NAA 0.5mg / L+KT 0.5mg / L+IBA 0.3mg / L+Vc 400~500mg / L+Sm (NO 3 ) 3 20~25mg / L;

[0026] 3. Proliferation culture:

[0027] Place the induced tissue in st...

specific Embodiment approach 2

[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the explant bud induction medium is MS+6-BA 1.5mg / L+NAA 0.5mg / L+KT 0.5mg / L+ IBA 0.3mg / L+Vc 450mg / L+Sm(NO 3 ) 3 20~25mg / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0032] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the described proliferation medium is MS+Sm (NO 3 ) 3 40mg / L+NAA 0.5mg / L+6-BA 0.5mg / L+IBA 0.5mg / L. Others are the same as in the first embodiment.

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Abstract

The invention relates to a propagation method of purple-leaf thick plum, which relates to a propagation method of purple-leaf thick plum. The method of the present invention is as follows: 1. explant selection; 2. explant bud induction; 3. proliferation cultivation; 4. rooting cultivation; The present invention increases the content of flavonoids, polyphenols and anthocyanins of Prunus aurantiae by adding Sm and Ce elements in the tissue culture medium, thereby improving the yield of the extract of A. Li's application value.

Description

technical field [0001] The invention relates to a method for propagating purple leaf thick plum. Background technique [0002] Prunus virginiana, also known as Canadian red cherry, is a deciduous tree of Rosaceae (Rosaceae). It is native to North America. It was selected from Prunus virginiana var.schubert in the 1950s. The plant height is 8~ 14m, bark taupe, branchlets smooth and open naturally, leaves luxuriant, leaves oblong to obovate, 8-14cm long, 5-7cm wide, margins with thick teeth, short branches blooming, leaf axils draw racemes, long inflorescences 4-6cm, white flowers, purple-red fruit in April-June flowering period, bitter taste, bright peel, well-developed root system in July-September fruiting period, strong suitability, like sunshine and warm and humid climate environment, fertile, loose and well-drained It grows robustly on sandy loam soil, has strong resistance to stress, is resistant to cold and drought, is resistant to barrenness, and is resistant to dise...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘洪家张久明
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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