Ultrasonic-microwave extraction process for total flavonoids in leaves of Ilex cornuta Lindl.ex Paxt.

An extraction process and technology of citrifolia leaf, applied in the field of ultrasonic-microwave extraction technology of total flavonoids from citrifolia leaf, can solve the problems of high total flavonoid impurities, large solvent consumption and residue, low extraction rate, etc., and achieves high product content and detection The effect of scientific method and simple process

CN105287662AInactive Publication Date: 2016-02-03JINING MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-02-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an ultrasonic-microwave extraction process for total flavonoids in leaves of Ilex cornuta Lindl.ex Paxt., and relates to the technical field of an ultrasonic-microwave extraction process, aiming at solving the problem of an existing method on the content of total flavonoids in leaves of Ilex cornuta Lindl.ex Paxt. According to the extraction process, through a single-factor experiment, the influence of seven factors, namely temperature, ethanol concentration, solid-to-liquid ratio, microwave time, extraction number of times, ultrasonic time and grinding particle size, on an extraction rate is investigated, and four factors, namely the temperature, the ethanol concentration, the solid-to-liquid ratio and the microwave time, are determined in an orthogonal experiment; the extraction method is simple and convenient in process, high in product content and is suitable for extraction of total flavonoids in leaves of Ilex cornuta Lindl.ex Paxt.; and a detection method, involving quality standard, is scientific, advanced, rapid and strong in operability.
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Description

Technical field

[0001] The invention relates to the field of ultrasonic-microwave extraction technology, in particular to the ultrasonic-microwave extraction process of total flavonoids from leaves of citrifolia. Background technique

[0002] The leaves are the dried leaves of Ilexcornuta Lindl.exPaxt., a plant of the family Ilexaceae. Harvest in autumn, remove impurities and sun-dried. This product is in the shape of a rectangular or rectangular mesh, and occasionally has a long oval mesh. Length 3 ~ 200px, width 1.5 ~ 100px. The apex has 3 large hard thorn teeth, the top 1 is often recurved, the base is truncated or broadly wedge-shaped, sometimes each side has 1 to 3 thorn teeth, and the edge is slightly revolute; the long oval leaves are often without thorns tooth. The upper surface is yellow-green or green-brown, shiny, and the lower surface is gray-yellow or gray-green. The veins are pinnate and the petioles are short. Leather. Hard and thick. Gas micro, slightl...

Examples

Embodiment Construction

[0030] The present invention will be further described below in conjunction with specific embodiments.

[0031] 1. Ultrasonic-microwave extraction process of total flavonoids from leaves of medusa, the extraction process comprising the following steps:

[0032] (1) get the cilantro leaf after drying in the shade and pulverize, obtain the powder of 80 meshes, take by weighing 5g of citrifolia leaf powder, for subsequent use;

[0033] (2) place 5g of the leaf powder of wolfberry that was weighed in step (1) into a 250ml conical flask, add the corresponding material-to-liquid ratio, the ethanol of the corresponding concentration, at the corresponding temperature, place it in an ultrasonic cleaning machine for ultrasonic 20min, extract 2 times;

[0034] (3) transfer all the solutions mixed with thorn bone powder obtained in step (2) into three-necked round-bottomed flasks, place them in a microcomputer microwave chemical reactor, and react for corresponding time;

[0035] (4) ta...