Preparation method of carboxymethylated catathelasma ventricosum polysaccharide

Abstract

The invention discloses a preparation method of carboxymethylated catathelasma ventricosum polysaccharide. The preparation method comprises the steps of freeze-drying catathelasma ventricosum, grinding into powder, performing water extraction and alcohol sedimentation with ethanol, and removing protein and small molecular weight impurities, so as to obtain catathelasma ventricosum polysaccharide; the preparation method of the carboxymethylated catathelasma ventricosum polysaccharide comprises the steps of dissolving 250 mg of catathelasma ventricosum polysaccharide in 10 ml of NaOH solution, fully stirring mixed liquid for 30 min, adding a mixed solution of 3 g of chloroacetic acid and 15 ml of isopropanol, stirring for 1 h, then putting in an incubator of 65 DEG C for reaction for 4 h, cooling to room temperature, then adjusting pH to be neutral with 0.5 M of HCl, dialyzing by running water overnight, wherein the molecular weight cutoff of a dialysis bag is 3000, mixing with 95% of ethanol solution in four times volume, stirring for 30 min, then putting in a refrigerator of 4 DEG C for 12 h, then centrifuging, discarding supernatant, and freeze-drying, so as to obtain the carboxymethylated catathelasma ventricosum polysaccharide.

Description

technical field [0001] The invention relates to the technical field of preparation of polysaccharides, in particular to a method for preparing carboxymethylated pine mushroom polysaccharides (cCVPs). Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. According to data released by the World Health Organization in 2014, the number of people with diabetes worldwide has reached 347 million. [0003] Edible fungi with medicinal value are considered as one of the very important therapeutic drugs in traditional folk medicine, and are widely used. Studies have shown that the treatment of diseases by edible fungi is mainly caused by the biologically active substances it c...

AI Technical Summary

Problems solved by technology

However, the carboxymethylation methods of polysaccharides are common at this stage: the degree of substitution of carboxymethylated polysaccharides is low, and when the reagent concentration and reaction time are increased, the yield of carboxymethylated polysaccharides will be seriously affected.

At present, there is no research on the carboxymethylation of polysaccharides from P. The activity is inversely proportional, so the application of carboxymethylated polysaccharides to anti-diabetic research will provide new ideas and references for the chemical modification of polysaccharides and the biological activity of polysaccharides

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