Spawn preparation method by switching enoki mushroom liquid spawn culture to solid spawn culture prior to liquification

A technology of liquid strain and reliquefaction, which is applied in the field of seed production of Flammulina velutipes from liquid strain to solid and reliquefaction, can solve the problems of high cost equipment investment, high technical requirements, low cost, etc., and achieve simple production and high infection rate of bacteria Low, pulp tender effect

Inactive Publication Date: 2016-03-02
JIANNGSU KANGSHENG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Among them, the liquid strain has many advantages such as short production cycle, consistent bacterial age, easy operation, and neat mushroom production. It is suitable for the factory standard production of Flammulina velutipes, but it also has the disadvantages of large equipment investment, high technical requirements, and many risks.
Although solid strains have many disadvantages, they have low cost, simple seed production process, and high strain stability, especially in strain purification, rejuvenation, fruiting test, strain preservation, and transportation.

Method used

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with embodiment.

[0022] The first step of the method of the present invention: cultivating liquid strains, the specific steps are as follows.

[0023] (1-1) Production of liquid culture medium: The ingredients required for each 1000ml of liquid triangular flask culture medium are: 20g of sugar, 3.3g of soybean milk treasure, 0.66g of potassium dihydrogen phosphate, and 0.66g of magnesium sulfate. Weigh all kinds of raw materials and mix them evenly ;Put the prepared material into a 1000ml triangular flask, stir and dissolve it fully, put it into a rotor, and then use pure water to set the volume to 600ml, and the pH value is required to be 6.2 to 6.5; plug the filled triangular bottle Put on a tampon, and cover the tampon with tinfoil to ensure that the tinfoil wraps the tampon completely, then put it in an autoclave, sterilize at 121°C for 60 minutes and take it out; put the taken out triangular fla...

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Abstract

The invention discloses a spawn preparation method by switching enoki mushroom liquid spawn culture to solid spawn culture prior to liquification. The spawn preparation method includes the steps of liquid spawn culture, solid spawn culture, solid spawn liquification and the like, so that a tedious production mode of adopting a fermentation tank in liquid spawn culture is abandoned; paper pulp serving as a solid culture raw material is prepared into granules, and the characteristic that lignicolous fungi need lignin is kept, so that a certain clearance degree among mycelia in a culture vessel is achieved, and the requirement of a mycelium colonization process on oxygen is met.

Description

technical field [0001] The invention relates to a seed production method for converting liquid strains of Flammulina velutipes into solids and then liquefying them, and belongs to the technical field of Flammulina velutipes cultivation. Background technique [0002] At present, in edible fungus enterprises, the use of strains is mainly divided into solid strains and liquid strains. Among them, the liquid strain has many advantages such as short production cycle, consistent bacterial age, easy operation, and neat mushroom production. It is suitable for the factory standard production of Flammulina velutipes, but it also has the disadvantages of large equipment investment, high technical requirements, and many risks. . Although solid strains have many disadvantages, they have low cost, simple seed production process, and high strain stability, especially in strain purification, rejuvenation, fruiting test, strain preservation, and transportation. They also have irreplaceable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 范伯圣朱志林厉芳张俊廖光阳池和彬丁虎
Owner JIANNGSU KANGSHENG AGRI DEV CO LTD
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