SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof

A small intestinal submucosa and patch technology, applied in medical science, prosthesis, etc., can solve the problems of short half-life, poor stability, and high requirements, and achieve strong bone regeneration ability, wide application prospects, and reliable composite materials.

Active Publication Date: 2016-03-23
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Drugs that promote bone regeneration required in bone tissue engineering have always been a research hotspot. Although BMP-2 protein is currently the most effective osteogenic induction factor that is widely recognized and used, it is very expensive, and the use of BMP gene introduction There are certain safety problems in the method, so its clinical use is still restricted by cost and safety.
In addition, BMP-2 and other protein drugs have the problems of poor stability and short half-life in vivo, correspondingly, the requirements for drug release systems are very high
However, there is no report on the loading of icariin on SIS as a sustained-release drug for promoting bone regeneration.

Method used

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  • SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof
  • SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof
  • SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The small intestinal submucosa drug-loaded patch of Example 1, its microstructure includes SIS collagen fiber skeleton and icariin loaded on the SIS collagen fiber skeleton, the SIS collagen fiber skeleton is a reticular fiber structure, and icariin is a particle shape (grain size is 0.3~0.6μm), icariin is attached to the SIS collagen fiber skeleton, and the adhesion amount of icariin is 33.2±5.6μg / cm 2 , its preparation method comprises the following steps:

[0025] 1) Preparation of the small intestinal submucosa patch: In a sterile environment, take out the dry and sterile SIS material, cut it into a small intestinal submucosa patch of 1 cm × 1 cm, and then wash the cut small intestinal submucosa patch with deionized water. Immerse for 12-24 hours to fully wet the material, discard the deionized water and soak with 50% ethanol solution for 1-2 hours, then replace with 70%, 80%, 90% ethanol solution and absolute ethanol in sequence after discarding the ethanol solutio...

Embodiment 2

[0028] The small intestinal submucosa drug-loaded patch of Example 2, its microstructure includes SIS collagen fiber skeleton and icariin loaded on the SIS collagen fiber skeleton, the SIS collagen fiber skeleton is a reticular fiber structure, and icariin is a particle shape (particle size: 0.3-5μm), icariin is attached to the SIS collagen fiber skeleton, and the adhesion amount of icariin is 56.2±8.8μg / cm 2 , its preparation method comprises the following steps:

[0029] 1) Preparation of the small intestinal submucosa patch: In a sterile environment, take out the dry and sterile SIS material, cut it into a small intestinal submucosa patch of 1 cm × 1 cm, and then wash the cut small intestinal submucosa patch with deionized water. Immerse for 12-24 hours to fully wet the material, discard the deionized water and soak with 50% ethanol solution for 1-2 hours, then replace with 70%, 80%, 90% ethanol solution and absolute ethanol in sequence after discarding the ethanol solution...

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Abstract

The invention discloses an SIS (small intestinal submucosa) medicine carrying patch. A microstructure of the SIS medicine carrying patch comprises an SIS collagenous fiber skeleton and icariin supported on the SIS collagenous fiber skeleton, wherein the SIS collagenous fiber skeleton adopts a reticular fiber structure; icariin is granular and attached to the SIS collagenous fiber skeleton, and the attaching amount of icariin is 20-80 mu g / cm <2>. According to the SIS medicine carrying patch, granular icariin is attached to the SIS collagenous fiber skeleton, a stable medicine release system is established, two potential bone tissue engineering elements, namely, the SIS collagenous fiber skeleton and icariin, are combined, formed SIS medicine carrying patch composite is reliable and stable, characteristics such as safety, economy, stable chemical property, excellent bone regeneration promoting capability, vascular regeneration promotion capability and the like of icariin are sufficiently utilized, the SIS medicine carrying patch has very high bone regeneration promoting capability and has a wide application prospect in the bone regeneration field, and the preparation method is simple.

Description

technical field [0001] The invention belongs to the technical field of drug release systems in biomedical engineering, and in particular relates to a small intestinal submucosa drug-loaded patch and its preparation method and application. Background technique [0002] About 3.5 million people in my country have bone defects due to different reasons every year, and about 1.5 million bone surgery transplants are performed. The demand for replacement bones is very large, especially for replacement bones for large bone injuries. With the rapid development of tissue engineering technology, constructing osteoconductive and osteoinductive artificial substitute bone in vitro is an important way to solve this demand. Scaffold materials play a crucial role in tissue engineered bone construction. Compared with other synthetic materials, natural extracellular matrix (ECM) materials have the following characteristics: 1. Excellent biocompatibility; 2. The three-dimensional structure tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/36A61L27/54
CPCA61L27/24A61L27/3629A61L27/365A61L27/54A61L2300/232A61L2300/602
Inventor 赵基源李梅谷俏俏陈梦洁陈松娣罗钫妙张迟
Owner NINGBO UNIV
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