Method for separating and enriching hematopoietic stem cells in peripheral blood
A technology for separating and enriching hematopoietic stem cells, which is applied to the field of separating and enriching hematopoietic stem cells from peripheral blood that is not mobilized by bone marrow, can solve the problems of bone marrow sampling trauma, unfavorable expansion and culture, and the number and purity of hematopoietic stem cells are not high, so as to solve the problem of obtaining materials. Inconvenient, pain-avoiding effects
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[0052] The present invention extracts HSC from non-mobilized peripheral blood, avoids the problems in the prior art, and opens up a new source.
[0053] In recent years, the development of HSC surface marker research has enriched the methods of stem cell separation and purification. According to the surface markers of HSC, hematopoietic stem cells can be detected by a variety of cell biology and molecular biology methods such as immunocytochemistry, flow cytometry, molecular hybridization, and PCR. As mentioned above, most of the hematopoietic stem cells are still selected based on the enrichment of CD34+ cells. For example, the purity of fluorescence activated cell sorting (FACS) can reach 95%, but the cost is higher and the instrument is also more expensive. Therefore, the present invention uses the immunoadsorption system-immunomagnetic bead to separate CD34+ cells, and immunomagnetic bead separation system for separation Large amount, separation purity and cell quality can b...
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