Method for making polypeptide chelated calcium with oil-peeled wood frog residual body as raw material

A technology of polypeptide chelated calcium and preparation method, applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problems of waste of resources, pollute the environment, etc., and achieve the effect of reducing emissions and improving economic and ecological benefits

Inactive Publication Date: 2016-03-23
JILIN UNIV
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Problems solved by technology

Most of them are discarded, which causes a great

Method used

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Embodiment Construction

[0012] The preparation method of the polypeptide chelated calcium provided by the invention with the wood frog residue after oil stripping as raw material, its specific method is as follows:

[0013] Step 1. Pretreatment of Rana residues: Grinding the Rana residues or male frogs from which the Rana oil has been removed to fine residues with a multi-functional pulverizer, passing through a 30-50 mesh sieve to obtain fine powder of Rana residues;

[0014] Step 2, enzymatic hydrolysis: Put 100g of the wood frog residue fine powder obtained in step 1 into a container, add 1000ml of deionized water and stir to dissolve, then add 1000ml of the same deionized water to make the volume to 2000ml, measure the pH value to 6.5, and stir evenly Finally, add 6g of neutral protease, continue to stir the solution evenly, then place it in an electric heating constant temperature water tank, set the enzymatic hydrolysis temperature at 50°C, and fully enzymatically hydrolyze for 9 hours to obtain...

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Abstract

The invention discloses a method for making polypeptide chelated calcium with an oil-peeled wood frog residual body as the raw material. The method includes the specific steps of 1, wood frog residual body pretreatment; 2, enzymolysis; 3, nanofiltration freezing and drying and 4, chelation. The method has the advantages that the urgent requirement for wood frog industry development can be met, and the 'recycling economy' requirement for the wood frog industry development is met. A great number of bioactive substances are obtained through waste resources by means of comprehensive utilization, deep processing of the resources is achieved, the purposes of thorough eating and squeezing are achieved, emission to the environment is reduced, economic benefits and ecological benefits of the food industry are increased, the utilization rate of the edible resources is close to 100%, and green, organic, biological and functional wood frog products can become the industry of the most potential for development.

Description

technical field [0001] The invention relates to a preparation method of polypeptide chelated calcium, in particular to a preparation method of polypeptide chelated calcium which takes oil-stripping wood frog residues as raw materials. Background technique [0002] At present, the processing of wood frogs is still mainly to obtain female frog oil, and the intensive processing and utilization of about 75% of the female frog bone and flesh residues and male frogs after stripping the frog oil is still blank. Most of them are discarded, which causes a great waste of resources and pollutes the environment. [0003] Peptide chelated calcium is a new generation of calcium supplement product, which has broad application prospects in the fields of food and medical care. It is a problem to be solved how to utilize the female frog flesh and blood after stripping the frog oil to make polypeptide chelated calcium. Contents of the invention [0004] The purpose of the present invention...

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K14/78
Inventor 关爽卢静于寒松
Owner JILIN UNIV
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