Sciatic nerve ligation subcutaneous tumor-bearing rat model and application of sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine

An anti-tumor drug and sciatic nerve technology, applied in the field of medicine, can solve problems such as the inconvenience of new drugs

Inactive Publication Date: 2016-04-06
上海市崇明新华癌痛转化研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there is no experimental animal model that can induce long-term stable pain and visually observe tumor growth, which brings a lot of inconvenience to the research of the above problems and the development of new drugs

Method used

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  • Sciatic nerve ligation subcutaneous tumor-bearing rat model and application of sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine
  • Sciatic nerve ligation subcutaneous tumor-bearing rat model and application of sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine
  • Sciatic nerve ligation subcutaneous tumor-bearing rat model and application of sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The specific steps for preparation of sciatic nerve ligation and subcutaneous tumor-bearing model rats are as follows:

[0040] 1. Prepare tumor cells: take out the concentration of 1×10 8 After the Walker256 cell cryopreservation tube with cells / ml, quickly put it in a 37℃ water bath to thaw. Aspirate 1ml of cell suspension with a pipette in the ultraclean workbench, transfer it into a centrifuge tube, centrifuge at 1200 rpm for 5 minutes, discard the supernatant, add an appropriate amount of saline to mix, and then centrifuge at the same speed for 5 minutes. Discard the supernatant liquid. Then add 1ml of physiological saline and mix well to form a cell suspension.

[0041] Three healthy female Wistar rats weighing 60-80g were injected intraperitoneally into each rat with 0.2ml of the above-mentioned Walker256 tumor cell suspension. After the rats were raised for 7 days, the rat's abdomen showed obvious bulging, indicating that the rat's ascites increased , Take out 2ml ...

Embodiment 2

[0050] Example 2: Observation of different doses of morphine hydrochloride to relieve spontaneous pain, mechanical pain, and thermal pain in rats

[0051] The invention can apply the rat model of sciatic nerve ligation with subcutaneous tumor-bearing to the preliminary test of new analgesic and anti-tumor drugs. For example, the drug to be tested is applied to the model rat in the required mode of administration and dosage, and the effects of the drug on spontaneous pain, mechanical pain, and thermal pain in the rat are observed, as well as the weight and tumor formation of the rat. Rate, the influence of tumor mass, etc. Take morphine hydrochloride as an example to demonstrate the application of this model in testing the analgesic and anti-tumor effects of new drugs. For other drugs to be screened, refer to the method in this example.

[0052] The 6th day after tumor cell implantation and sciatic nerve ligation was selected as the detection time point, 5 mg / kg and 10 mg / kg of m...

Embodiment 3

[0057] Example 3: Effect of continuous administration of different doses of morphine hydrochloride on weight change, tumor formation rate, and tumor volume in tumor-bearing rats

[0058] From the 5th day after the tumor cells were implanted in the rat and the sciatic nerve ligation was completed, 5 mg / kg and 10 mg / kg of morphine hydrochloride were injected intraperitoneally into the sciatic nerve ligation subcutaneous tumor-bearing model rats. Normal saline was used as a control reagent daily Two injections were injected for 7 consecutive days. On the 12th day, the weight and tumor volume of the rats were measured, and the effect of morphine injection on the tumor formation rate was tested.

[0059] Morphine hydrochloride of 5 mg / kg and 10 mg / kg was administered intraperitoneally twice a day for 7 consecutive days, and physiological saline was used as a control. Observe the change of rat's body weight on the 12th day after tumor cell implantation, Picture 10 The results show that...

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Abstract

The invention relates to a sciatic nerve ligation subcutaneous tumor-bearing rat model and application of the sciatic nerve ligation subcutaneous tumor-bearing rat model in screening of analgesic and anti-tumor medicine. Single-side sciatic nerve ligation is conducted on an adult Wistar rat subjected to hypodermic implantation of Walker256 tumor cell suspension, the pain degree of the rat is reflected through spontaneous pain, the mechanical pain threshold value and the hot pain threshold value, and the tumor growth situation is indicated through the body weight change, the tumor formation rate and the tumor size of the rat. After the to-be-detected medicine is applied to the animal model according to the required mode and dosage, and the effect of the to-be-detected medicine on the aspect of analgesia and tumor prevention is judged by observing changes of the indexes. The experimental animal model which has the lasting and stable pain characteristic and can visually measure the tumor growth situation of the tumor of the rat is used for exploring the mechanism of promoting tumor growth through pain and used for screening the novel analgesic and anti-tumor medicine. The new carrier is provided for research and development of the novel analgesic and anti-tumor medicine.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a sciatic nerve ligation subcutaneous tumor-bearing rat model and its application in screening analgesic and antitumor drugs. Background technique [0002] Clinical research shows that about 20%-50% of cancer patients suffer from pain, and more than 75%-90% of advanced cancer patients are accompanied by severe pain symptoms. The existence of cancer pain has become another intractable chronic pain that plagues human beings after inflammatory pain and neuropathic pain. Severe pain can lead to insomnia, depression, weakness, anorexia and other symptoms, seriously affecting the quality of life of patients . More and more clinical studies suggest that cancer pain should be regarded as a disease that seriously threatens the quality of life and even survival of patients, no matter from the perspective of cancer pain itself or as a complication of tumors. . DanH et al. fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A01K67/02
CPCA01K67/02A61K49/0008
Inventor 丁罡姜峰叶品胡薇薇李静
Owner 上海市崇明新华癌痛转化研究所
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