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Utricularia aurea rapid breeding method

A kind of yellow castellae, rapid technology, applied in the field of plant reproduction, to achieve the effect of shortening the breeding cycle of seedlings, rapid reproduction, and realizing large-scale production

Inactive Publication Date: 2016-04-13
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no tissue culture technique report for the yellow raccoon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the method for the rapid propagation of Utricula algae of the present invention comprises the following steps:

[0015] (1) Selection and disinfection of explants: take the branches and leaves of U. chrysalis as explants, soak them in 2v / v% detergent solution for 5min, wash them with linear tap water for 15-30min, add 2-3 drops of Sterilize 100 ml of 0.1v / v% mercuric chloride at temperature -20 for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain explants, in which sterile water is sterilized by high temperature and high pressure of distilled water,

[0016] (2) Obtain the explants obtained in step (1) of sterile test-tube plantlets and inoculate them into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, at a culture temperature of 23-27°C , the light intensity is 1500lux, and the light time is 8-10 hours / day under the conditions of cultivating for 30 days to obtain st...

Embodiment 2

[0020] An example of the method for the rapid propagation of Utricula algae of the present invention comprises the following steps:

[0021] (1) Selection and disinfection of explants: take the branches and leaves of U. chrysalis as explants, soak them in 2v / v% detergent solution for 5min, wash them with linear tap water for 15-30min, add 2-3 drops of Sterilize 100 ml of 0.1v / v% mercuric chloride at temperature -20 for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain explants, in which sterile water is sterilized by high temperature and high pressure of distilled water,

[0022] (2) Obtain the explants obtained in step (1) of sterile test-tube plantlets and inoculate them into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, at a culture temperature of 23-27°C , the light intensity is 1500lux, and the light time is 8-10 hours / day under the conditions of cultivating for 30 days to obtain st...

Embodiment 3

[0026] An example of the method for the rapid propagation of Utricula algae of the present invention comprises the following steps:

[0027] (1) Selection and disinfection of explants: take the branches and leaves of U. chrysalis as explants, soak them in 2v / v% detergent solution for 5min, wash them with linear tap water for 15-30min, add 2-3 drops of Sterilize 100 ml of 0.1v / v% mercuric chloride at temperature -20 for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain explants, in which sterile water is sterilized by high temperature and high pressure of distilled water,

[0028] (2) Obtain the explants obtained in step (1) of sterile test-tube plantlets and inoculate them into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, at a culture temperature of 23-27°C , the light intensity is 1500lux, and the light time is 8-10 hours / day under the conditions of cultivating for 30 days to obtain st...

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Abstract

A utricularia aurea rapid propagation method comprises the following steps: (1) taking utricularia aurea branches and leaves as explants for disinfection; (2) inoculating the disinfected explants onto a MS + 1.0mg / L6-BA + 0.5mg / LNAA medium for induction germination to obtain sterile test-tube plantlets; (3) placing the sterile test-tube plantlets individually into a propagation medium for rapid propagation culture of the test-tube plantlets to obtain a large number of test-tube plantlets; and (4) hardening the test-tube plantlets, and transplanting the test-tube plantlets into a water plant water cluster box. By the utricularia aurea rapid propagation method, the propagation coefficient of the utricularia aurea test-tube plantlets can reach 30-50 times, survival rate of the utricularia aurea test-tube plantlets transplanted to the water plant water cluster box reaches 95-98%, and the utricularia aurea scale breeding problem can be effectively solved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for rapid propagation of Utricula yellowiana. Background technique [0002] Utricularia aurea Lour. is an annual herbaceous plant belonging to the Utricularia family. It mostly grows in paddy fields or shallow water in still ponds. Planting in areas with three-dimensional greening or water surface greening on large water bodies can increase the landscape diversity of water bodies. But its cultivation is difficult, requiring clean water and no pollution. In order to prevent the extinction of wild U. chrysalis resources and ensure the sustainable utilization of U. chrysalis resources, it is necessary to conduct research on the seedling breeding technology of U. chrysalis to ensure sufficient seedlings for large-scale use in garden water landscapes. In the prior art, there is no report on the tissue culture technique of the yellow raccoon. Contents of the invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 李林轩韦坤华韦范缪剑华李翠韦莹王一诺肖东
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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