Target Cell-Specific Fusion Proteins and Vaccine Compositions as Porcine Reproductive and Respiratory Syndrome Virus Vaccine Antigens
A technology of respiratory syndrome and vaccine composition, which is applied in the field of target cell-specific fusion protein subunit vaccines, and can solve problems such as secondary infection, loss of protective efficacy of attenuated live vaccines, and easy damage to the host immune system
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[0052] 1. Construction of target cell-specific fusion protein expression plasmid, pBPAC-CM5RM5
[0053] The present invention first uses the forward primer F1 such as SEQ ID NO: 3 and the reverse primer R1 such as SEQ ID NO: 4 to determine the amino acid sequence of the adenylate cyclase of Bordetella whose N-terminal enzyme domain is deleted After the corresponding nucleic acid sequence was selected and amplified from Bordetella genomic DNA by PCR, KpnI site and NheI site were introduced at its 5' end and 3' end, respectively, to obtain the BPAC nucleic acid sequence such as SEQ ID NO: 5 ;
[0054] Then the BPAC nucleic acid sequence was subcloned into the recombinant expression plasmid pAmp-LacZ (purchased from Haomin Biotechnology Co., Ltd.) through the KpnI site and the NheI site. The nucleic acid sequence of the plasmid pAmp-LacZ is shown in SEQ ID NO: 13 ; In the process, PCR verification can be carried out by forward primer F1 and reverse primer reverse primer R1. The...
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