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Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force

A technology of light-induced dielectrophoresis and acquisition method, which is applied in the field of label-free cell electrical characteristics acquisition based on light-induced dielectrophoresis mechanical force, can solve problems such as unreported, and achieve the effect of high batch size and high throughput

Inactive Publication Date: 2016-04-13
SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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Problems solved by technology

At present, the real-time acquisition method of cell electrical characteristics with label-free and high-throughput characteristics has not been reported

Method used

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  • Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force
  • Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force
  • Method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0025] The present invention integrates opto-mechanical automation technology into life science, utilizes digital programmable incident light generated and controlled by a computer, and projects it onto the optoelectronic tweezers chip through a projector to stimulate and induce a virtual non-uniform electric field in space; The interaction of cells in the cell produces light-induced dielectrophoretic mechanical force; since the applied AC voltage of different frequencies will cause different characteristics of cell translation behavior, and at the same time, different types of cells have different polarization mechanisms under the action of an electric field. Therefore, with the help of The change of the frequency of the external AC voltage and the use of light-induced dielectrophoretic mechanical force can realize the non-destructive, label-free and personal...

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Abstract

The invention relates to a method for acquiring label-free cells electrical characteristics based on light-induced dielectrophoresis mechanical force. The method comprises the following steps: incident light is projected to a lower surface of a photoelectron tweezer chip; frequency of alternating voltage emitted by a signal-generator is affected on a conducting layer of the photoelectron tweezer chip; cells move far from the incident light; frequency of applied alternating voltage can be increased, when certain cell is stationary, the frequency is a cross-over frequency of the cell; frequency of the applied alternating voltage is continuously increased, the cell moves towards to the incident light; when all the cells move in an incident light scope, the incident light and the applied alternating voltage are closed; a relation between the cross-over frequency and a diameter of each cell can be obtained; and capacitance of each cell membrane can be obtained according to the relation between the cell membrane capacitance and cell diameter. The preparation of metal microelectrodes with a specific structure is not required; the electrical characteristic parameter of single cell or many cells can be obtained with real time; an antibody is not required for being used for identification of the cells, and the method has the advantages of automation, mass production and high throughput capacity.

Description

technical field [0001] The invention relates to the application of light-mechanical-electrical coupling robot technology in life sciences, in particular to a non-destructive and label-free cell electrical characteristic acquisition method based on light-induced dielectrophoretic mechanical force. Background technique [0002] The cell membrane capacitance is closely related to the electrophysiological characteristics of the cell itself. The change of the cell membrane capacitance reflects the change of the cell membrane thickness, the composition of the cell membrane and the size of the cell itself, and the thickness and composition of the cell membrane are closely related to the type, state and shape of the cell. Therefore, the detection of the cell membrane capacitance is not only Different types of cells can be distinguished, as well as cells in different states, such as cancerous and normal cells, different types of cells, perforated cells and normal cells, differentiated...

Claims

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Application Information

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IPC IPC(8): G01N33/574
Inventor 刘连庆梁文峰李文荣张伟京肖秀斌
Owner SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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