Application of polypeptide hydrogel serving as protein vaccine adjuvant and protein vaccine

A protein vaccine and hydrogel technology, applied in the field of protein vaccines, can solve problems such as difficulty in large-scale preparation, achieve good adjuvant effect, and increase the variety of effects

Pending Publication Date: 2016-04-20
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology needs to covalently link the antigen to the self-assembling polypeptide, and it is not easy to prepare on a large scale.

Method used

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  • Application of polypeptide hydrogel serving as protein vaccine adjuvant and protein vaccine
  • Application of polypeptide hydrogel serving as protein vaccine adjuvant and protein vaccine
  • Application of polypeptide hydrogel serving as protein vaccine adjuvant and protein vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) According to Document 1, the L-configuration phosphorylated polypeptide Nap-GFFpY-OMe was synthesized by FMOC-solid-phase synthesis method, and its nuclear magnetic spectrum and mass spectrometry results are as follows:

[0021] 1 HNMR (400MHz, DMSO-d 6 )δ8.48(d,1H,NH),8.19-8.28(m,2H,NH),8.06(d,1H,NH),7.75-7.88(m,3H),7.75(s,1H),7.41- 7.48(m,3H),7.07-7.25(m,14H),4.46-4.58(m,3H),3.70-3.76(m,1H),3.6-3.68(s,4H),3.58(s,3H), 2.92-3.03(m,4H),2.76-2.81(m,1H),2.63-2.69(m,1H).MS:calc.M + =794.3, obsvd.(M+Na) + = 817.3.

[0022] (2) Take 1mgL configuration phosphorylated polypeptide Nap-GFFpY-OMe and place it in a 1.5ml glass bottle, add 150 microliters of PBS (pH=7.0) solution, adjust its pH value to 7.0 with sodium carbonate solution, at 20 Add 2 microliters of 2U / microliter alkaline phosphatase at ~25°C, set the volume to 250 microliters with PBS solution, let it stand for about 10 minutes to form a gel, and wait until the reaction is basically complete (about 2 hours)...

Embodiment 2

[0025] (1) Use the amino acid raw material of D configuration instead, according to literature 1, synthesize D configuration phosphorylated polypeptide Nap-GFFpY-OMe with FMOC-solid-phase synthesis method, its nuclear magnetic spectrum and mass spectrometry results are as follows:

[0026] 1 HNMR (300MHz, DMSO-d 6 )δ8.50(d,1H,NH),8.16-8.24(m,2H,NH),8.10(d,1H,NH),7.72-7.85(m,3H),7.74(s,1H),7.40- 7.46(m,3H),7.08-7.24(m,14H),4.47-4.59(m,3H),3.72-3.75(m,1H),3.58-3.68(s,4H),3.60(s,3H), 2.90-3.04(m,4H),2.73-2.85(m,1H),2.62-2.68(m,1H).MS:calc.M+=794.3,obsvd.(M+H) + =795.3.

[0027] (2) Take 1 mg D-configuration phosphorylated polypeptide Nap-GFF p Put Y-OMe in a 1.5 ml glass bottle, add 150 microliters of PBS (pH=7.0) solution, adjust the pH value to 7.0 with sodium carbonate solution, add 2 microliters of 2U / microliter at 20-25°C Alkaline phosphatase was fixed to 250 microliters with PBS solution, gelled after standing for about 10 minutes, and the polypeptide hydrogel was obta...

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Abstract

The invention provides an application of polypeptide hydrogel serving as a protein vaccine adjuvant and a protein vaccine. The hydrogel has good biocompatibility and can stimulate an organism to produce antigen-specific body fluid and cellular immune response very well after physically mixed with a protein antigen simply. According to the application, the polypeptide sequence is Nap-GFFY-OMe.

Description

technical field [0001] The invention relates to the application of a polypeptide hydrogel as a protein vaccine adjuvant and the protein vaccine. Background technique [0002] Hydrogels formed by self-assembled polypeptides have the characteristics and advantages of good biocompatibility and high biological activity. Research in related fields has been a hot spot in the past 20 years. It has shown high application prospects in regenerative medicine, drug controlled release, analysis and detection, tissue engineering, etc. For example, hydrogels formed by self-assembling polypeptides can be applied to the directed differentiation of stem cells, the preparation of cartilage tissues, the controlled release of anticancer drugs, etc. Recently, Professor Joel Collier of the University of Chicago found that self-assembling polypeptides can be used as vaccine adjuvants for proteins. They covalently linked self-assembling polypeptides with short peptide antigens or protein antigens, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/00
CPCA61K39/39A61K39/0005A61K2039/55516
Inventor 杨志谋王玲杨成彪王怀民
Owner NANKAI UNIV
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