Application of CD166 as liver cancer diagnosis serum marker and kit thereof

A diagnostic kit, 1. The technology of CD166, which is applied in the field of clinical diagnosis, can solve the problem of high recurrence rate in the high-expression group, and achieve the effects of high specificity and sensitivity, convenient sampling, and easy detection

Inactive Publication Date: 2016-06-01
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

4. The recurrence rate of the CD166 high expression group is higher, and CD166 is related to the recur

Method used

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  • Application of CD166 as liver cancer diagnosis serum marker and kit thereof
  • Application of CD166 as liver cancer diagnosis serum marker and kit thereof
  • Application of CD166 as liver cancer diagnosis serum marker and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 Experimental materials

[0032] The kit was purchased from Shanghai Lichen Biotechnology Co., Ltd., full name of the kit: humanCD166ELISAKIT, article number: LC9012, composition: microwell enzyme plate 12 wells * 8 strips, standard (4ng / ml) 0.6ml, standard dilution 6ml , 6ml of sample diluent, detection antibody-HRP, 25ml of washing buffer, substrate A, substrate B, stop solution, sealing film, instructions, ziplock bag.

[0033] 2. Experimental method

[0034] The detection method of CD166 in serum samples is: double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA) method. Specific steps: (1) Prepare the washing buffer before the experiment, and equilibrate the ELISA plate at room temperature for 20 minutes. (2) Set standard wells, sample wells, and blank wells (add nothing). Add 50ul of standard substances of different concentrations to the standard wells; touch the conditions in advance to the sample wells, and dilute the serum samples 5 time...

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Abstract

The invention relates to an application of CD166 used as a liver cancer diagnosis serum marker and a kit thereof. It is firstly confirmed that CD166 can be used as a marker for liver cancer diagnosis. Thus, liver cancer disease can be diagnosed and predicted by detecting concentration of CD166 in serum. Sampling is convenient, and detection is easy. In addition, the invention has advantages of specificity, high sensitivity and the like. As CD166 is cell surface molecule, CD166 can be used for diagnosing liver cancer and is expected to become a molecular target for treating liver cancer.

Description

technical field [0001] The invention relates to the technical field of clinical diagnosis, in particular to a kit for diagnosing liver cancer and its application. Background technique [0002] Liver cancer is a malignant tumor of the liver. It is a common and frequently-occurring disease in surgical diseases. Liver cancer can be divided into two categories: primary and secondary. Primary malignant tumors of the liver originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer, which is a malignant tumor with a high incidence in my country and is extremely harmful; rare. Secondary or metastatic liver cancer refers to malignant tumors originating from multiple organs throughout the body and invading the liver. Generally, it is more common in liver metastases of malignant tumors in stomach, biliary tract, pancreas, colorectum, ovary, uterus, lung, breast and other organs. Since the liver is the largest substantial organ of the ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/574G01N33/68
Inventor 孙奋勇王佳谊
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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