Preparation method of para hydroxybenzene alanine urine detection reagent

A technology of hydroxyphenylalanine and detection reagents, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., which can solve the problem of unreliable and inapplicable statistical results. To solve the problems of tumor screening and the small number of specimens to be tested, to achieve the effect of fast detection, low cost and simple operation

Inactive Publication Date: 2017-01-25
程玉慧
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Problems solved by technology

The reagents provided by the above-mentioned prior art have certain effects in application, high specificity and sensitivity, but there are still deficiencies. The reagents provided by Chinese patent CN1096374 have a

Method used

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Experimental program
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Example Embodiment

[0018] Example 1

[0019] A liquid preparation: according to the volume ratio of 1:3, take the ninhydrin and the mass fraction of 80% ethanol solution and mix evenly, and then prepare for use; B liquid preparation: the molar ratio of 1:2:1.2, take formic acid, ethylene glycol and silver nitrate, Stir evenly, then add 30% ethylene glycol volume sterile water to dilute to obtain a colorless transparent liquid, ready for use; C solution preparation: according to mass ratio 10:1:3, take potassium iodide and azobisisobutamidine hydrochloride And 1.2mol / L platinum nitrate solution, mix well, then add 30% sterile water of the mixture mass, and place it at 50℃ for 20min, for use; combine the A solution prepared above, the prepared solution B and the prepared solution The C solution is mixed evenly according to the volume ratio of 2:1.2:0.6 to obtain the target product p-hydroxyphenylalanine urine test reagent.

[0020] Detection method: Take 8mL of fresh morning urine after fasting in the...

Example Embodiment

[0023] Example 2

[0024] A liquid preparation: according to the volume ratio of 1:3.5, take the ninhydrin and 80% ethanol solution and mix it evenly, and reserve; B liquid preparation: the molar ratio of 1:2.5:1.6, take formic acid, ethylene glycol and nitric acid Stir the silver evenly, then add 35% ethylene glycol volume sterile water to dilute to obtain a colorless transparent liquid, ready for use; C solution preparation: according to the mass ratio of 13:1:4, take potassium iodide and azobisisobutyramidine salt Salt and 1.2mol / L platinum nitrate solution, mix evenly, then add 32% sterile water of the mixture mass, and put it at 55℃ for 25min, standby; prepared liquid A, prepared liquid B and prepared The C solution is mixed evenly according to the volume ratio of 2.5:1.5:0.7 to obtain the target product p-hydroxyphenylalanine urine test reagent.

[0025] Detection method: Take 16mL of fresh morning urine after fasting in the morning and no use of Chinese and Western medicine...

Example Embodiment

[0028] Example 3

[0029] A solution preparation: according to the volume ratio of 1:4, take the ninhydrin and the mass fraction of 80% ethanol solution and mix it evenly, and reserve; B solution preparation: the molar ratio of 1:3:1.8, take formic acid, ethylene glycol and silver nitrate, Stir evenly, then add 40% ethylene glycol volume sterile water to dilute to obtain a colorless transparent liquid, ready for use; C liquid preparation: according to the mass ratio of 16:1:5, take potassium iodide and azobisisobutamidine hydrochloride And 1.2mol / L platinum nitrate solution, mix well, then add 35% sterile water of the mixture mass, and place it at 60℃ for 30min, for use; combine the prepared A liquid, the prepared B liquid and the prepared C solution is mixed evenly according to the volume ratio of 3:1.8:0.9 to obtain the target product p-hydroxyphenylalanine urine test reagent.

[0030] Detection method: Take 20mL of fresh morning urine after fasting in the morning and no use of ...

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Abstract

The invention relates to an in-vitro detection reagent for clinical diagnosis, and in particular to a preparation method of a para hydroxybenzene alanine urine detection reagent. The para hydroxybenzene alanine urine detection reagent provided by the invention contains silver ions, iodide ions, nitrate ions, platinum ions and azo bis-isobutyric amidine hydrochloride, and is prepared from mixing the components according to the proportion. The para hydroxybenzene alanine urine detection reagent provided by the invention is easy to sample and simple to operate, can be used for general survey of large-scale tumor metabolic disturbance and the like, is high in spectrum property and high in stability and sensitivity, has the ability of displaying specific color and a white precipitation phenomenon, has a better recognition effect, and is fast in detection speed.

Description

technical field [0001] The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a preparation method of a p-hydroxyphenylalanine urine detection reagent. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of death that threatens human life and health. According to statistics, the number of cancer patients will double every 25 years. Not only that, the number of deaths from cancer is also rising rapidly around the world, and this number may increase to 13.2 million in 2030. Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with adv...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/82
CPCG01N21/78G01N21/82
Inventor 程玉慧
Owner 程玉慧
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