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Formula of inducing medium for tomato anther culture breeding

A technology of induction medium and medium formulation, applied in the field of agricultural science, can solve problems such as gaps, unclear interaction between medium components and culture conditions, low embryoid body or callus induction rate and differentiation rate, etc.

Active Publication Date: 2016-06-15
新沂市芭缇雅商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The interaction between medium components and culture conditions is unclear, and the induction rate and differentiation rate of embryoid bodies or callus are low, which is far from the actual application requirements.

Method used

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  • Formula of inducing medium for tomato anther culture breeding
  • Formula of inducing medium for tomato anther culture breeding
  • Formula of inducing medium for tomato anther culture breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Prepare the culture medium with the following formula:

[0016] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, AgNO 3 8.0mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;

[0017] 2,4-D1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH5 .8.

[0018] Tomato cultivars Dayezaofen and Momotaro were selected as donors for anther culture, and flower buds in full bloom were taken at 9:00-11:00 am on a sunny day, and the flower buds were washed with washing powder to clean...

Embodiment 2

[0021] Prepare the medium with the following formula (AgNO 3 Adding the preferred concentration):

[0022]KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3 g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;

[0023] 2,4-D1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH5 .8.

[0024] AgNO 3 The added concentration is set in the following five types: 0; 2.0mg / L; 4.0mg / L; 6.0mg / L; 10.0mg / L.

[0025] The two tomato varieties were also selected as anther donors, the operation method...

Embodiment 3

[0027] Prepare the medium with the following formula (the preferred concentration of glycine added):

[0028] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, AgNO 3 8.0mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L L, sucrose 15g / L, plant gel 5.0g / L;

[0029] 2,4-D1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH5 .8.

[0030] The added concentration of glycine is set in the following four types: 0; 1.0mg / L; 3.0mg / L; 4.0mg / L.

[0031] The two tomato varieties were also selected as anther donors, the operation method and cu...

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Abstract

The invention provides a formula of an inducing medium for tomato anther culture breeding. According to the formula, the inducing medium is prepared from KNO3, NH4NO3, KH2PO4, MgSO4.H2O, CaCl2.2H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.H2O, ZnSO4.7H2O, H3BO3, KI, CuSO4.5H2O, CoCl.6H2O, NaMoO4.2H2O, AgNO3, inositol, vitamin B1, vitamin B6, nicotinic acid, glycine, folic acid, cyclic guanosine monophosphate, maltose, cane sugar, phytagel, 2,4-D, kinetin, complex phthalein nucleic acid, biotin, vitamin C, active carbon and protein hydrolysate at a proper proportion. The medium has the advantage of efficiently inducing tomato anther to generate pollen calli, and can significantly improve the tomato anther culture efficiency and quicken the tomato improved variety breeding progress.

Description

technical field [0001] The invention relates to a formula of induction medium for tomato flower cultivation, in particular to a formula of induction medium for significantly improving tomato anther healing rate, and belongs to the field of agricultural science and technology. Background technique [0002] Tomato, also known as tomato, belongs to the genus Solanaceae in classification and is an annual or perennial crop. Tomato is listed as one of the 30 crops with the highest annual output in the world, is one of the most common fruits and vegetables cultivated in the world, and is also one of the main cultivated vegetables in my country. Tomato has strong adaptability, high yield, rich fruit nutrition, rich in vitamins and sugars, has special flavor and wide application, and can adopt a variety of cultivation methods in the production field. In recent years, it has gradually developed into a worldwide crop. [0003] Due to the importance of tomato application, the research ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张瑞明
Owner 新沂市芭缇雅商贸有限公司
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